Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkab379
Title: Dengue virus 2 capsid protein chaperones the strand displacement of 5 '-3 ' cyclization sequences
Authors: Yong, Xin Ee 
Palur, V Raghuvamsi
Anand, Ganesh S 
Wohland, Thorsten 
Sharma, Kamal K 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
DEPENDENT RNA-POLYMERASE
NUCLEIC-ACID CHAPERONE
CIS-ACTING REPLICATION
VIRAL-RNA
NUCLEOCAPSID PROTEIN
IN-VITRO
NEGATIVE-STRAND
CODING REGION
CORE PROTEIN
3'-TERMINAL REGIONS
Issue Date: 25-May-2021
Publisher: OXFORD UNIV PRESS
Citation: Yong, Xin Ee, Palur, V Raghuvamsi, Anand, Ganesh S, Wohland, Thorsten, Sharma, Kamal K (2021-05-25). Dengue virus 2 capsid protein chaperones the strand displacement of 5 '-3 ' cyclization sequences. NUCLEIC ACIDS RESEARCH 49 (10) : 5832-5844. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkab379
Abstract: By virtue of its chaperone activity, the capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements. However, the role of DENV2C during the interaction of RNA elements involved in stabilizing the 5′-3′ panhandle structure of DENV RNA is still unclear. Therefore, we determined how DENV2C affects structural functionality of the capsid-coding region hairpin element (cHP) during annealing and strand displacement of the 9-nt cyclization sequence (5CS) and its complementary 3CS. cHP has two distinct functions: a role in translation start codon selection and a role in RNA synthesis. Our results showed that cHP impedes annealing between 5CS and 3CS. Although DENV2C does not modulate structural functionality of cHP, it accelerates annealing and specifically promotes strand displacement of 3CS during 5′-3′ panhandle formation. Furthermore, DENV2C exerts its chaperone activity by favouring one of the active conformations of cHP. Based on our results, we propose mechanisms for annealing and strand displacement involving cHP. Thus, our results provide mechanistic insights into how DENV2C regulates RNA synthesis by modulating essential RNA elements in the capsid-coding region, that in turn allow for DENV replication.
Source Title: NUCLEIC ACIDS RESEARCH
URI: https://scholarbank.nus.edu.sg/handle/10635/226581
ISSN: 0305-1048
1362-4962
DOI: 10.1093/nar/gkab379
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