Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkab379
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dc.titleDengue virus 2 capsid protein chaperones the strand displacement of 5 '-3 ' cyclization sequences
dc.contributor.authorYong, Xin Ee
dc.contributor.authorPalur, V Raghuvamsi
dc.contributor.authorAnand, Ganesh S
dc.contributor.authorWohland, Thorsten
dc.contributor.authorSharma, Kamal K
dc.date.accessioned2022-06-07T01:59:21Z
dc.date.available2022-06-07T01:59:21Z
dc.date.issued2021-05-25
dc.identifier.citationYong, Xin Ee, Palur, V Raghuvamsi, Anand, Ganesh S, Wohland, Thorsten, Sharma, Kamal K (2021-05-25). Dengue virus 2 capsid protein chaperones the strand displacement of 5 '-3 ' cyclization sequences. NUCLEIC ACIDS RESEARCH 49 (10) : 5832-5844. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkab379
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/226581
dc.description.abstractBy virtue of its chaperone activity, the capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements. However, the role of DENV2C during the interaction of RNA elements involved in stabilizing the 5′-3′ panhandle structure of DENV RNA is still unclear. Therefore, we determined how DENV2C affects structural functionality of the capsid-coding region hairpin element (cHP) during annealing and strand displacement of the 9-nt cyclization sequence (5CS) and its complementary 3CS. cHP has two distinct functions: a role in translation start codon selection and a role in RNA synthesis. Our results showed that cHP impedes annealing between 5CS and 3CS. Although DENV2C does not modulate structural functionality of cHP, it accelerates annealing and specifically promotes strand displacement of 3CS during 5′-3′ panhandle formation. Furthermore, DENV2C exerts its chaperone activity by favouring one of the active conformations of cHP. Based on our results, we propose mechanisms for annealing and strand displacement involving cHP. Thus, our results provide mechanistic insights into how DENV2C regulates RNA synthesis by modulating essential RNA elements in the capsid-coding region, that in turn allow for DENV replication.
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectDEPENDENT RNA-POLYMERASE
dc.subjectNUCLEIC-ACID CHAPERONE
dc.subjectCIS-ACTING REPLICATION
dc.subjectVIRAL-RNA
dc.subjectNUCLEOCAPSID PROTEIN
dc.subjectIN-VITRO
dc.subjectNEGATIVE-STRAND
dc.subjectCODING REGION
dc.subjectCORE PROTEIN
dc.subject3'-TERMINAL REGIONS
dc.typeArticle
dc.date.updated2022-06-06T23:34:40Z
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentBIOLOGY (NU)
dc.description.doi10.1093/nar/gkab379
dc.description.sourcetitleNUCLEIC ACIDS RESEARCH
dc.description.volume49
dc.description.issue10
dc.description.page5832-5844
dc.published.statePublished
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