Please use this identifier to cite or link to this item: https://doi.org/10.1042/BSR20201707
Title: A 12-week aerobic exercise intervention results in improved metabolic function and lower adipose tissue and ectopic fat in high-fat diet fed rats
Authors: Gopalan, Venkatesh
Yaligar, Jadegoud 
Michael, Navin
Kaur, Kavita
Anantharaj, Rengaraj 
Verma, Sanjay Kumar
Sadananthan, Suresh Anand 
Le, Giang Thi Thu
Goh, Jorming 
Velan, S Sendhil
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
POLYMERASE CHAIN-REACTION
LIFE-STYLE INTERVENTION
SKELETAL-MUSCLE
INSULIN-RESISTANCE
LIPID-METABOLISM
GENE-EXPRESSION
ENZYME-ACTIVITY
LIVER-DISEASE
OBESITY
ADAPTATIONS
Issue Date: 1-Jan-2021
Publisher: PORTLAND PRESS LTD
Citation: Gopalan, Venkatesh, Yaligar, Jadegoud, Michael, Navin, Kaur, Kavita, Anantharaj, Rengaraj, Verma, Sanjay Kumar, Sadananthan, Suresh Anand, Le, Giang Thi Thu, Goh, Jorming, Velan, S Sendhil (2021-01-01). A 12-week aerobic exercise intervention results in improved metabolic function and lower adipose tissue and ectopic fat in high-fat diet fed rats. BIOSCIENCE REPORTS 41 (1). ScholarBank@NUS Repository. https://doi.org/10.1042/BSR20201707
Abstract: Investigations of long-term exercise interventions in humans to reverse obesity is expensive and is hampered by poor compliance and confounders. In the present study, we investigated intrahepatic and muscle fat, visceral and subcutaneous fat pads, plasma metabolic profile and skeletal muscle inflammatory markers in response to 12-week aerobic exercise in an obese rodent model. Six-week-old male Wistar rats (n=20) were randomized to chow-fed control (Control, n=5), sedentary high-fat diet (HFD, n=5), chow-fed exercise (Exercise, n=5) and HFD-fed exercise (HFD+Exercise, n=5) groups. The exercise groups were subjected to 12 weeks of motorized treadmill running at a speed of 18 m/min for 30 min/day. Differences in post-intervention measures were assessed by analysis of covariance (ANCOVA), adjusted for baseline bodyweight and pre-intervention measures, where available. Post-hoc analyses were performed with Bonferroni correction. Plasma metabolic profile was worsened and fat pads, ectopic fat in muscle and liver and inflammatory markers in skeletal muscle were elevated in sedentary HFD-fed animals relative to chow-fed controls. HFD+Exercise animals had significantly lower leptin (P=0.0004), triglycerides (P=0.007), homeostatic model assessment of insulin resistance (HOMA-IR; P=0.065), intramyocellular lipids (IMCLs; P=0.003), intrahepatic lipids (IHLs; P<0.0001), body fat% (P=0.001), subcutaneous adipose tissue (SAT; P<0.0001), visceral adipose (P<0.0001) and total fat mass (P<0.0001), relative to sedentary HFD-fed animals, despite only modestly lower bodyweight. Messenger RNA (mRNA) expression of inflammatory markers Interleukin 6 (IL6) and Tumor necrosis factor α (TNFα) were also reduced with aerobic exercise in skeletal muscle. Our results suggest that 12 weeks of aerobic exercise training is effective in improving metabolic health, fat depots, ectopic fat and inflammation even against a high-fat dietary background.
Source Title: BIOSCIENCE REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/219440
ISSN: 0144-8463
1573-4935
DOI: 10.1042/BSR20201707
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