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https://doi.org/10.1371/journal.pone.0221305
Title: | Detection and prognostic relevance of circulating tumour cells (CTCs) in Asian breast cancers using a label-free microfluidic platform | Authors: | Yap, Yoon-Sim Leong, Man Chun Chua, Yong Wei Loh, Kiley Wei Jen Lee, Guek Eng Lim, Elaine Hsuen Dent, Rebecca Ng, Raymond Chee Hui Lim, John Heng-Chi Singh, Garima Tan, Angela Guan, Guofeng Wu, Andrew Lee, Yi Fang Bhagat, Ali Asgar S Lim, Darren Wan-Teck |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics CLINICAL ONCOLOGY/COLLEGE AMERICAN SOCIETY ENRICHMENT ESTROGEN RECOMMENDATIONS PROGRESSION SURVIVAL |
Issue Date: | 25-Sep-2019 | Publisher: | PUBLIC LIBRARY SCIENCE | Citation: | Yap, Yoon-Sim, Leong, Man Chun, Chua, Yong Wei, Loh, Kiley Wei Jen, Lee, Guek Eng, Lim, Elaine Hsuen, Dent, Rebecca, Ng, Raymond Chee Hui, Lim, John Heng-Chi, Singh, Garima, Tan, Angela, Guan, Guofeng, Wu, Andrew, Lee, Yi Fang, Bhagat, Ali Asgar S, Lim, Darren Wan-Teck (2019-09-25). Detection and prognostic relevance of circulating tumour cells (CTCs) in Asian breast cancers using a label-free microfluidic platform. PLOS ONE 14 (9). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0221305 | Abstract: | Objectives We aimed to study the prevalence of CTCs in breast cancer (BC) patients undergoing neoadjuvant or palliative therapy with a label-free microfluidic platform (ClearCell FX), and its prognostic relevance in metastatic BC (mBC). Materials and methods Peripheral blood samples were collected from 108 BC patients before starting a new line of treatment (“baseline”), majority of whom had mBC (76/108; 70.4%). CTCs were retrieved by dean flow fractionation that enriched for larger cells, and enumerated using immunofluorescence-based staining. Progression-free survival (PFS) in mBC patients was analysed using Kaplan-Meier method; cox proportional hazard models were used for univariable and multivariable analyses. Results The detection rate of CTCs before starting a new line of treatment was 75.9% (n = 108; median: 8 CTCs/7.5 ml blood) at a cut off of ≥2 CTCs. PFS was inferior for mBC patients with baseline CTC count ≥5 CTCs/7.5 ml blood vs. those with < 5 CTCs/7.5 ml blood (median PFS: 4.3 vs. 7.0 months; p-value: 0.037). The prognostic relevance of CTCs was most significant in patients with HER2- mBC (median PFS: 4.1 vs. 8.3 months; p-value: 0.032), luminal (HR+HER2-) subtype (median PFS: 4.2 vs. 8.3 months; p-value: 0.048), and patients who had one or more prior treatments (median PFS: 4.2 vs. 7.0 months; p-value: 0.02). On multivariable analysis, baseline CTC level (hazard ratio (HR): 1.84, p-value: 0.02) and pre-treatment status (HR: 1.87, p-value: 0.05) were independent predictors of PFS. Conclusions This work demonstrates the prognostic significance of CTCs in mBC detected using a label-free size-based enrichment platform. | Source Title: | PLOS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/218596 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0221305 |
Appears in Collections: | Elements Staff Publications |
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