Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41551-017-0181-y
Title: Engineered commensal microbes for diet-mediated colorectal-cancer chemoprevention
Authors: Ho, Chun Loong 
Tan, Hui Qing 
Chua, Koon Jiew 
Kang, Aram
Lim, Kiat Hon 
Ling, Khoon Lin 
Yew, Wen Shan
Lee, Yung Seng 
Thiery, Jean Paul 
Chang, Matthew Wook 
Keywords: Science & Technology
Technology
Engineering, Biomedical
Engineering
ALLYL-ISOTHIOCYANATE
ANTICANCER ACTIVITY
SULFORAPHANE
CELLS
MECHANISMS
MYROSINASE
STABILITY
ADJUVANT
BROCCOLI
Issue Date: 1-Jan-2018
Publisher: NATURE PUBLISHING GROUP
Citation: Ho, Chun Loong, Tan, Hui Qing, Chua, Koon Jiew, Kang, Aram, Lim, Kiat Hon, Ling, Khoon Lin, Yew, Wen Shan, Lee, Yung Seng, Thiery, Jean Paul, Chang, Matthew Wook (2018-01-01). Engineered commensal microbes for diet-mediated colorectal-cancer chemoprevention. NATURE BIOMEDICAL ENGINEERING 2 (1) : 27-37. ScholarBank@NUS Repository. https://doi.org/10.1038/s41551-017-0181-y
Abstract: Chemoprevention - the use of medication to prevent cancer - can be augmented by the consumption of produce enriched with natural metabolites. However, chemopreventive metabolites are typically inactive and have low bioavailability and poor host absorption. Here, we show that engineered commensal microbes can prevent carcinogenesis and promote the regression of colorectal cancer through a cruciferous vegetable diet. The engineered commensal Escherichia coli bound specifically to the heparan sulphate proteoglycan on colorectal cancer cells and secreted the enzyme myrosinase to transform hostingested glucosinolates - natural components of cruciferous vegetables - to sulphoraphane, an organic small molecule with known anticancer activity. The engineered microbes coupled with glucosinolates resulted in >95% proliferation inhibition of murine, human and colorectal adenocarcinoma cell lines in vitro. We also show that murine models of colorectal carcinoma fed with the engineered microbes and the cruciferous vegetable diet displayed significant tumour regression and reduced tumour occurrence.
Source Title: NATURE BIOMEDICAL ENGINEERING
URI: https://scholarbank.nus.edu.sg/handle/10635/216102
ISSN: 2157846X
DOI: 10.1038/s41551-017-0181-y
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