Please use this identifier to cite or link to this item:
Title: Transfer-matrix calculations of the effects of tension and torque constraints on DNA-protein interactions
Authors: Efremov, A.K. 
Yan, J. 
Issue Date: 2018
Publisher: Oxford University Press
Citation: Efremov, A.K., Yan, J. (2018). Transfer-matrix calculations of the effects of tension and torque constraints on DNA-protein interactions. Nucleic Acids Research 46 (13) : 6504-6527. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: Organization and maintenance of the chromosomal DNA in living cells strongly depends on the DNA interactions with a plethora of DNA-binding proteins. Single-molecule studies show that formation of nucleoprotein complexes on DNA by such proteins is frequently subject to force and torque constraints applied to the DNA. Although the existing experimental techniques allow to exert these type of mechanical constraints on individual DNA biopolymers, their exact effects in regulation of DNA-protein interactions are still not completely understood due to the lack of systematic theoretical methods able to efficiently interpret complex experimental observations. To fill this gap, we have developed a general theoretical framework based on the transfer-matrix calculations that can be used to accurately describe behaviour of DNA-protein interactions under force and torque constraints. Potential applications of the constructed theoretical approach are demonstrated by predicting how these constraints affect the DNAbinding properties of different types of architectural proteins.Obtained results provide important insights into potential physiological functions of mechanical forces in the chromosomal DNA organization by architectural proteins as well as into single-DNA manipulation studies of DNA-protein interactions. © The Author(s) 2018.
Source Title: Nucleic Acids Research
ISSN: 0305-1048
DOI: 10.1093/nar/gky478
Rights: Attribution-NonCommercial 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1093_nar_gky478.pdf1.93 MBAdobe PDF



Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons