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Title: Single-cell analysis of circulating tumor cells: Why heterogeneity matters
Authors: Lim, S.B.
Lim, C.T. 
Lim, W.-T. 
Keywords: Cellular heterogeneity
Circulating tumor cells
Single-cell analysis
Issue Date: 2019
Publisher: MDPI AG
Citation: Lim, S.B., Lim, C.T., Lim, W.-T. (2019). Single-cell analysis of circulating tumor cells: Why heterogeneity matters. Cancers 11 (10) : 1595. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Unlike bulk-cell analysis, single-cell approaches have the advantage of assessing cellular heterogeneity that governs key aspects of tumor biology. Yet, their applications to circulating tumor cells (CTCs) are relatively limited, due mainly to the technical challenges resulting from extreme rarity of CTCs. Nevertheless, recent advances in microfluidics and immunoaffinity enrichment technologies along with sequencing platforms have fueled studies aiming to enrich, isolate, and sequence whole genomes of CTCs with high fidelity across various malignancies. Here, we review recent single-cell CTC (scCTC) sequencing efforts, and the integrated workflows, that have successfully characterized patient-derived CTCs. We examine how these studies uncover DNA alterations occurring at multiple molecular levels ranging from point mutations to chromosomal rearrangements from a single CTC, and discuss their cellular heterogeneity and clinical consequences. Finally, we highlight emerging strategies to address key challenges currently limiting the translation of these findings to clinical practice. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Cancers
ISSN: 20726694
DOI: 10.3390/cancers11101595
Rights: Attribution 4.0 International
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