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Title: Microhexagon gradient array directs spatial diversification of spinal motor neurons
Authors: Lim, G.S.
Hor, J.H.
Ho, N.R.Y.
Wong, C.Y.
Ng, S.Y. 
Soh, B.S. 
Shao, H. 
Keywords: Microfluidics
Molecular gradient
Motor neuron
Spinal cord
Stem cell
Issue Date: 2019
Publisher: Ivyspring International Publisher
Citation: Lim, G.S., Hor, J.H., Ho, N.R.Y., Wong, C.Y., Ng, S.Y., Soh, B.S., Shao, H. (2019). Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9 (2) : 311-323. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: Motor neuron diversification and regionalization are important hallmarks of spinal cord development and rely on fine spatiotemporal release of molecular cues. Here, we present a dedicated platform to engineer complex molecular profiles for directed neuronal differentiation. Methods: The technology, termed microhexagon interlace for generation of versatile and fine gradients (microHIVE), leverages on an interlocking honeycomb lattice of microstructures to dynamically pattern molecular profiles at a high spatial resolution. By packing the microhexagons as a divergent, mirrored array, the platform not only enables maximal mixing efficiency but also maintains a small device footprint. Results: Employing the microHIVE platform, we developed optimized profiles of growth factors to induce rostral-caudal patterning of spinal motor neurons, and directed stem cell differentiation in situ into a spatial continuum of different motor neuron subtypes. Conclusions: The differentiated cells showed progressive RNA and protein signatures, consistent with that of representative brachial, thoracic and lumbar regions of the human spinal cord. The microHIVE platform can thus be utilized to develop advanced biomimetic systems for the study of diseases in vitro. © Ivyspring International Publisher.
Source Title: Theranostics
ISSN: 18387640
DOI: 10.7150/thno.29755
Rights: Attribution-NonCommercial 4.0 International
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