Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-019-11332-4
Title: Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages
Authors: Chua, A.C.Y.
Ong, J.J.Y.
Malleret, B. 
Suwanarusk, R.
Kosaisavee, V. 
Zeeman, A.-M.
Cooper, C.A.
Tan, K.S.W. 
Zhang, R. 
Tan, B.H.
Abas, S.N.
Yip, A.
Elliot, A.
Joyner, C.J.
Cho, J.S.
Breyer, K.
Baran, S.
Lange, A.
Maher, S.P.
Nosten, F.
Bodenreider, C.
Yeung, B.K.S.
Mazier, D.
Galinski, M.R.
Dereuddre-Bosquet, N.
Le Grand, R.
Kocken, C.H.M.
Rénia, L. 
Kyle, D.E.
Diagana, T.T.
Snounou, G.
Russell, B.
Bifani, P. 
Issue Date: 2019
Publisher: Nature Publishing Group
Citation: Chua, A.C.Y., Ong, J.J.Y., Malleret, B., Suwanarusk, R., Kosaisavee, V., Zeeman, A.-M., Cooper, C.A., Tan, K.S.W., Zhang, R., Tan, B.H., Abas, S.N., Yip, A., Elliot, A., Joyner, C.J., Cho, J.S., Breyer, K., Baran, S., Lange, A., Maher, S.P., Nosten, F., Bodenreider, C., Yeung, B.K.S., Mazier, D., Galinski, M.R., Dereuddre-Bosquet, N., Le Grand, R., Kocken, C.H.M., Rénia, L., Kyle, D.E., Diagana, T.T., Snounou, G., Russell, B., Bifani, P. (2019). Robust continuous in vitro culture of the Plasmodium cynomolgi erythrocytic stages. Nature Communications 10 (1) : 3635. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-11332-4
Rights: Attribution 4.0 International
Abstract: The ability to culture pathogenic organisms substantially enhances the quest for fundamental knowledge and the development of vaccines and drugs. Thus, the elaboration of a protocol for the in vitro cultivation of the erythrocytic stages of Plasmodium falciparum revolutionized research on this important parasite. However, for P. vivax, the most widely distributed and difficult to treat malaria parasite, a strict preference for reticulocytes thwarts efforts to maintain it in vitro. Cultivation of P. cynomolgi, a macaque-infecting species phylogenetically close to P. vivax, was briefly reported in the early 1980s, but not pursued further. Here, we define the conditions under which P. cynomolgi can be adapted to long term in vitro culture to yield parasites that share many of the morphological and phenotypic features of P. vivax. We further validate the potential of this culture system for high-throughput screening to prime and accelerate anti-P. vivax drug discovery efforts. © 2019, The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/210713
ISSN: 20411723
DOI: 10.1038/s41467-019-11332-4
Rights: Attribution 4.0 International
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