Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms20092140
Title: Effect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction
Authors: Spannbauer, A.
Traxler, D.
Lukovic, D.
Zlabinger, K.
Winkler, J.
Gugerell, A.
Ferdinandy, P.
Hausenloy, D.J. 
Pavo, N.
Emmert, M.Y.
Hoerstrup, S.P.
Jakab, A.
Gyöngyösi, M.
Riesenhuber, M.
Keywords: Acute myocardial infarction
Cardiac electrophysiology
Electrocardiography
Exosome-rich fraction
Exosomes
Extracellular vesicles
Ischemia-reperfusion injury
Ischemic postconditioning
Ischemic preconditioning
Mirna
Reperfusion arrhythmia
Ventricular arrhythmia
Issue Date: 2019
Publisher: MDPI AG
Citation: Spannbauer, A., Traxler, D., Lukovic, D., Zlabinger, K., Winkler, J., Gugerell, A., Ferdinandy, P., Hausenloy, D.J., Pavo, N., Emmert, M.Y., Hoerstrup, S.P., Jakab, A., Gyöngyösi, M., Riesenhuber, M. (2019). Effect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction. International Journal of Molecular Sciences 20 (9) : 2140. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms20092140
Rights: Attribution 4.0 International
Abstract: We investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) and measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion of themid left anterior coronary artery, followed by reperfusion. The animalswere divided into three groups: acutemyocardial infarction (AMI, n = 7), ischemic preconditioning-acutemyocardial infarction (IPC-AMI) (n = 9), orAMI-PostC (n = 5). IPCwas induced by three 5-min. episodes of repetitive ischemia/reperfusion cycles (rI/R) before AMI. PostC was induced by six 30-s rI/R immediately after induction of reperfusion 90 min after occlusion. Before the angiographic procedure, a NOGA endocardial mapping catheter was placed again the distal anterior ventricular endocardium to record the intracardiac electrogram (R-amplitude, ST-Elevation, ST-area under the curve (AUC), QRS width, and corrected QT time (QTc)) during the entire procedure. An arrhythmia score was calculated. Cardiac MRI was performed afterone-month. IPC led to significantly lower ST-elevation, heart rate, and arrhythmia score during ischemia. PostC induced a rapid recovery of R-amplitude, decrease in QTc, and lower arrhythmia score during reperfusion. Slightly higher levels ofmiR-26 andmiR-133 were observed in AMI compared to groups IPC-AMI and AMI-PostC. Significantly lower levels of miR-1, miR-208, and miR-328 were measured in the AMI-PostC group as compared to animals in group AMI and IPC-AMI. The arrhythmia score was not significantly associated with miRNA plasma levels. CardiacMRI showed significantly smaller infarct size in the IPC-AMI group when compared to the AMI and AMI-PostC groups. Thus, IPC led to better left ventricular ejection fraction at one-month and it exerted antiarrhythmic effects during ischemia, whereas PostC exhibited antiarrhythmic properties after reperfusion, with significant downregulaton of ischemia-related miRNAs. © 2019 by the authors.
Source Title: International Journal of Molecular Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/209981
ISSN: 1661-6596
DOI: 10.3390/ijms20092140
Rights: Attribution 4.0 International
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