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Title: | Effect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction | Authors: | Spannbauer, A. Traxler, D. Lukovic, D. Zlabinger, K. Winkler, J. Gugerell, A. Ferdinandy, P. Hausenloy, D.J. Pavo, N. Emmert, M.Y. Hoerstrup, S.P. Jakab, A. Gyöngyösi, M. Riesenhuber, M. |
Keywords: | Acute myocardial infarction Cardiac electrophysiology Electrocardiography Exosome-rich fraction Exosomes Extracellular vesicles Ischemia-reperfusion injury Ischemic postconditioning Ischemic preconditioning Mirna Reperfusion arrhythmia Ventricular arrhythmia |
Issue Date: | 2019 | Publisher: | MDPI AG | Citation: | Spannbauer, A., Traxler, D., Lukovic, D., Zlabinger, K., Winkler, J., Gugerell, A., Ferdinandy, P., Hausenloy, D.J., Pavo, N., Emmert, M.Y., Hoerstrup, S.P., Jakab, A., Gyöngyösi, M., Riesenhuber, M. (2019). Effect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction. International Journal of Molecular Sciences 20 (9) : 2140. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms20092140 | Rights: | Attribution 4.0 International | Abstract: | We investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) and measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion of themid left anterior coronary artery, followed by reperfusion. The animalswere divided into three groups: acutemyocardial infarction (AMI, n = 7), ischemic preconditioning-acutemyocardial infarction (IPC-AMI) (n = 9), orAMI-PostC (n = 5). IPCwas induced by three 5-min. episodes of repetitive ischemia/reperfusion cycles (rI/R) before AMI. PostC was induced by six 30-s rI/R immediately after induction of reperfusion 90 min after occlusion. Before the angiographic procedure, a NOGA endocardial mapping catheter was placed again the distal anterior ventricular endocardium to record the intracardiac electrogram (R-amplitude, ST-Elevation, ST-area under the curve (AUC), QRS width, and corrected QT time (QTc)) during the entire procedure. An arrhythmia score was calculated. Cardiac MRI was performed afterone-month. IPC led to significantly lower ST-elevation, heart rate, and arrhythmia score during ischemia. PostC induced a rapid recovery of R-amplitude, decrease in QTc, and lower arrhythmia score during reperfusion. Slightly higher levels ofmiR-26 andmiR-133 were observed in AMI compared to groups IPC-AMI and AMI-PostC. Significantly lower levels of miR-1, miR-208, and miR-328 were measured in the AMI-PostC group as compared to animals in group AMI and IPC-AMI. The arrhythmia score was not significantly associated with miRNA plasma levels. CardiacMRI showed significantly smaller infarct size in the IPC-AMI group when compared to the AMI and AMI-PostC groups. Thus, IPC led to better left ventricular ejection fraction at one-month and it exerted antiarrhythmic effects during ischemia, whereas PostC exhibited antiarrhythmic properties after reperfusion, with significant downregulaton of ischemia-related miRNAs. © 2019 by the authors. | Source Title: | International Journal of Molecular Sciences | URI: | https://scholarbank.nus.edu.sg/handle/10635/209981 | ISSN: | 1661-6596 | DOI: | 10.3390/ijms20092140 | Rights: | Attribution 4.0 International |
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