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dc.titleEffect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction
dc.contributor.authorSpannbauer, A.
dc.contributor.authorTraxler, D.
dc.contributor.authorLukovic, D.
dc.contributor.authorZlabinger, K.
dc.contributor.authorWinkler, J.
dc.contributor.authorGugerell, A.
dc.contributor.authorFerdinandy, P.
dc.contributor.authorHausenloy, D.J.
dc.contributor.authorPavo, N.
dc.contributor.authorEmmert, M.Y.
dc.contributor.authorHoerstrup, S.P.
dc.contributor.authorJakab, A.
dc.contributor.authorGyöngyösi, M.
dc.contributor.authorRiesenhuber, M.
dc.identifier.citationSpannbauer, A., Traxler, D., Lukovic, D., Zlabinger, K., Winkler, J., Gugerell, A., Ferdinandy, P., Hausenloy, D.J., Pavo, N., Emmert, M.Y., Hoerstrup, S.P., Jakab, A., Gyöngyösi, M., Riesenhuber, M. (2019). Effect of ischemic preconditioning and postconditioning on exosome-rich fraction microrna levels, in relation with electrophysiological parameters and ventricular arrhythmia in experimental closed-chest reperfused myocardial infarction. International Journal of Molecular Sciences 20 (9) : 2140. ScholarBank@NUS Repository.
dc.description.abstractWe investigated the antiarrhythmic effects of ischemic preconditioning (IPC) and postconditioning (PostC) by intracardiac electrocardiogram (ECG) and measured circulating microRNAs (miRs) that are related to cardiac conduction. Domestic pigs underwent 90-min. percutaneous occlusion of themid left anterior coronary artery, followed by reperfusion. The animalswere divided into three groups: acutemyocardial infarction (AMI, n = 7), ischemic preconditioning-acutemyocardial infarction (IPC-AMI) (n = 9), orAMI-PostC (n = 5). IPCwas induced by three 5-min. episodes of repetitive ischemia/reperfusion cycles (rI/R) before AMI. PostC was induced by six 30-s rI/R immediately after induction of reperfusion 90 min after occlusion. Before the angiographic procedure, a NOGA endocardial mapping catheter was placed again the distal anterior ventricular endocardium to record the intracardiac electrogram (R-amplitude, ST-Elevation, ST-area under the curve (AUC), QRS width, and corrected QT time (QTc)) during the entire procedure. An arrhythmia score was calculated. Cardiac MRI was performed afterone-month. IPC led to significantly lower ST-elevation, heart rate, and arrhythmia score during ischemia. PostC induced a rapid recovery of R-amplitude, decrease in QTc, and lower arrhythmia score during reperfusion. Slightly higher levels ofmiR-26 andmiR-133 were observed in AMI compared to groups IPC-AMI and AMI-PostC. Significantly lower levels of miR-1, miR-208, and miR-328 were measured in the AMI-PostC group as compared to animals in group AMI and IPC-AMI. The arrhythmia score was not significantly associated with miRNA plasma levels. CardiacMRI showed significantly smaller infarct size in the IPC-AMI group when compared to the AMI and AMI-PostC groups. Thus, IPC led to better left ventricular ejection fraction at one-month and it exerted antiarrhythmic effects during ischemia, whereas PostC exhibited antiarrhythmic properties after reperfusion, with significant downregulaton of ischemia-related miRNAs. © 2019 by the authors.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.sourceScopus OA2019
dc.subjectAcute myocardial infarction
dc.subjectCardiac electrophysiology
dc.subjectExosome-rich fraction
dc.subjectExtracellular vesicles
dc.subjectIschemia-reperfusion injury
dc.subjectIschemic postconditioning
dc.subjectIschemic preconditioning
dc.subjectReperfusion arrhythmia
dc.subjectVentricular arrhythmia
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.sourcetitleInternational Journal of Molecular Sciences
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