Please use this identifier to cite or link to this item: https://doi.org/10.1049/enb.2018.5007
Title: Characterisation of Constitutive Promoters from the Anderson library in Chromobacterium violaceum ATCC 12472
Authors: LIOW LU TING 
MAYBELLE DARLENE KHO GO 
YEW WEN SHAN 
Issue Date: Sep-2019
Publisher: Institution of Engineering and Technology (IET)
Citation: LIOW LU TING, MAYBELLE DARLENE KHO GO, YEW WEN SHAN (2019-09). Characterisation of Constitutive Promoters from the Anderson library in Chromobacterium violaceum ATCC 12472. Engineering Biology 3 (3) : 57-66. ScholarBank@NUS Repository. https://doi.org/10.1049/enb.2018.5007
Abstract: Chromobacterium violaceum is a potential industrially relevant chassis due to its cyanogenic properties and diverse metabolic energetics. However, its application in synthetic biology is limited by the lack of characterised genetic parts. Six constitutive promoters of a 100-fold range in gene expression from the Anderson library were characterised in C. violaceum using an automated microplate reader. The datasheet provides an insight to the highly similar trend in promoter strengths in C. violaceum to the original characterised strengths of the Anderson library constitutive promoters in E. coli. This provides a foundation for constitutive promoters of different strengths as parts to be used for synthetic biology gene constructs in C. violaceum.
Source Title: Engineering Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/188801
ISSN: 2398-6182
2398-6182
DOI: 10.1049/enb.2018.5007
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