Characterisation of Constitutive Promoters from the Anderson library in Chromobacterium violaceum ATCC 12472

Abstract

Chromobacterium violaceum is a potential industrially relevant chassis due to its cyanogenic properties and diverse metabolic energetics. However, its application in synthetic biology is limited by the lack of characterised genetic parts. Six constitutive promoters of a 100-fold range in gene expression from the Anderson library were characterised in C. violaceum using an automated microplate reader. The datasheet provides an insight to the highly similar trend in promoter strengths in C. violaceum to the original characterised strengths of the Anderson library constitutive promoters in E. coli. This provides a foundation for constitutive promoters of different strengths as parts to be used for synthetic biology gene constructs in C. violaceum.