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Title: Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells
Authors: Caminschi, I
Ahmet, F
Heger, K
Brady, J
Nutt, S.L
Vremec, D
Pietersz, S
Lahoud, M.H
Schofield, L
Hansen, D.S
O'Keeffe, M
Smyth, M.J
Bedoui, S
Davey, G.M
Villadangos, J.A
Heath, W.R
Shortman, K 
Keywords: antigen
major histocompatibility antigen class 2
natural killer cell receptor
RAG2 protein
transcription factor PU 1
animal cell
animal model
antigen presenting cell
cell function
cell proliferation
cell stimulation
cell structure
controlled study
cytokine production
dendritic cell
interferon producing killer dendritic cell
natural killer cell
priority journal
protein expression
T lymphocyte
Antigens, CD45
Dendritic Cells
Histocompatibility Antigens Class II
Integrin alpha2
Killer Cells, Natural
Lymphocyte Activation
Issue Date: 2007
Citation: Caminschi, I, Ahmet, F, Heger, K, Brady, J, Nutt, S.L, Vremec, D, Pietersz, S, Lahoud, M.H, Schofield, L, Hansen, D.S, O'Keeffe, M, Smyth, M.J, Bedoui, S, Davey, G.M, Villadangos, J.A, Heath, W.R, Shortman, K (2007). Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells. Journal of Experimental Medicine 204 (11) : 2579-2590. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Interferon-producing killer dendritic cells (IKDCs) have been described as possessing the lytic potential of NK cells and the antigen-presenting capacity of dendritic cells (DCs). In this study, we examine the lytic function and antigen-presenting capacity of mouse spleen IKDCs, including those found in DC preparations. IKDCs efficiently killed NK cell targets, without requiring additional activation stimuli. However, in our hands, when exposed to protein antigen or to MHC class II peptide, IKDCs induced little or no T cell proliferation relative to conventional DCs or plasmacytoid DCs, either before or after activation with CpG, or in several disease models. Certain developmental features indicated that IKDCs resembled NK cells more than DCs. IKDCs, like NK cells, did not express the transcription factor PU.1 and were absent from recombinase activating gene-2-null, common ?-chain-null (Rag2 -/-Il2rg-/-) mice. When cultured with IL-15 and -18, IKDCs proliferated extensively, like NK cells. Under these conditions, a proportion of expanded IKDCs and NK cells expressed high levels of surface MHC class II. However, even such MHC class II+ IKDCs and NK cells induced poor T cell proliferative responses compared with DCs. Thus, IKDCs resemble NK cells functionally, and neither cell type could be induced to be effective antigen-presenting cells. JEM © The Rockefeller University Press.
Source Title: Journal of Experimental Medicine
ISSN: 0022-1007
DOI: 10.1084/jem.20071351
Rights: Attribution 4.0 International
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