Please use this identifier to cite or link to this item:
https://doi.org/10.1084/jem.20071351
DC Field | Value | |
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dc.title | Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells | |
dc.contributor.author | Caminschi, I | |
dc.contributor.author | Ahmet, F | |
dc.contributor.author | Heger, K | |
dc.contributor.author | Brady, J | |
dc.contributor.author | Nutt, S.L | |
dc.contributor.author | Vremec, D | |
dc.contributor.author | Pietersz, S | |
dc.contributor.author | Lahoud, M.H | |
dc.contributor.author | Schofield, L | |
dc.contributor.author | Hansen, D.S | |
dc.contributor.author | O'Keeffe, M | |
dc.contributor.author | Smyth, M.J | |
dc.contributor.author | Bedoui, S | |
dc.contributor.author | Davey, G.M | |
dc.contributor.author | Villadangos, J.A | |
dc.contributor.author | Heath, W.R | |
dc.contributor.author | Shortman, K | |
dc.date.accessioned | 2020-10-27T06:57:48Z | |
dc.date.available | 2020-10-27T06:57:48Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Caminschi, I, Ahmet, F, Heger, K, Brady, J, Nutt, S.L, Vremec, D, Pietersz, S, Lahoud, M.H, Schofield, L, Hansen, D.S, O'Keeffe, M, Smyth, M.J, Bedoui, S, Davey, G.M, Villadangos, J.A, Heath, W.R, Shortman, K (2007). Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells. Journal of Experimental Medicine 204 (11) : 2579-2590. ScholarBank@NUS Repository. https://doi.org/10.1084/jem.20071351 | |
dc.identifier.issn | 0022-1007 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/181030 | |
dc.description.abstract | Interferon-producing killer dendritic cells (IKDCs) have been described as possessing the lytic potential of NK cells and the antigen-presenting capacity of dendritic cells (DCs). In this study, we examine the lytic function and antigen-presenting capacity of mouse spleen IKDCs, including those found in DC preparations. IKDCs efficiently killed NK cell targets, without requiring additional activation stimuli. However, in our hands, when exposed to protein antigen or to MHC class II peptide, IKDCs induced little or no T cell proliferation relative to conventional DCs or plasmacytoid DCs, either before or after activation with CpG, or in several disease models. Certain developmental features indicated that IKDCs resembled NK cells more than DCs. IKDCs, like NK cells, did not express the transcription factor PU.1 and were absent from recombinase activating gene-2-null, common ?-chain-null (Rag2 -/-Il2rg-/-) mice. When cultured with IL-15 and -18, IKDCs proliferated extensively, like NK cells. Under these conditions, a proportion of expanded IKDCs and NK cells expressed high levels of surface MHC class II. However, even such MHC class II+ IKDCs and NK cells induced poor T cell proliferative responses compared with DCs. Thus, IKDCs resemble NK cells functionally, and neither cell type could be induced to be effective antigen-presenting cells. JEM © The Rockefeller University Press. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | antigen | |
dc.subject | interferon | |
dc.subject | major histocompatibility antigen class 2 | |
dc.subject | natural killer cell receptor | |
dc.subject | RAG2 protein | |
dc.subject | transcription factor PU 1 | |
dc.subject | animal cell | |
dc.subject | animal model | |
dc.subject | antigen presenting cell | |
dc.subject | article | |
dc.subject | cell function | |
dc.subject | cell proliferation | |
dc.subject | cell stimulation | |
dc.subject | cell structure | |
dc.subject | controlled study | |
dc.subject | cytokine production | |
dc.subject | dendritic cell | |
dc.subject | female | |
dc.subject | interferon producing killer dendritic cell | |
dc.subject | mouse | |
dc.subject | natural killer cell | |
dc.subject | nonhuman | |
dc.subject | phenotype | |
dc.subject | priority journal | |
dc.subject | protein expression | |
dc.subject | T lymphocyte | |
dc.subject | Animals | |
dc.subject | Antigens, CD45 | |
dc.subject | Dendritic Cells | |
dc.subject | Histocompatibility Antigens Class II | |
dc.subject | Immunophenotyping | |
dc.subject | Integrin alpha2 | |
dc.subject | Interferons | |
dc.subject | Killer Cells, Natural | |
dc.subject | Lymphocyte Activation | |
dc.subject | Mice | |
dc.subject | Spleen | |
dc.subject | T-Lymphocytes | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.description.doi | 10.1084/jem.20071351 | |
dc.description.sourcetitle | Journal of Experimental Medicine | |
dc.description.volume | 204 | |
dc.description.issue | 11 | |
dc.description.page | 2579-2590 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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