Please use this identifier to cite or link to this item: https://doi.org/10.1084/jem.20071351
DC FieldValue
dc.titlePutative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells
dc.contributor.authorCaminschi, I
dc.contributor.authorAhmet, F
dc.contributor.authorHeger, K
dc.contributor.authorBrady, J
dc.contributor.authorNutt, S.L
dc.contributor.authorVremec, D
dc.contributor.authorPietersz, S
dc.contributor.authorLahoud, M.H
dc.contributor.authorSchofield, L
dc.contributor.authorHansen, D.S
dc.contributor.authorO'Keeffe, M
dc.contributor.authorSmyth, M.J
dc.contributor.authorBedoui, S
dc.contributor.authorDavey, G.M
dc.contributor.authorVilladangos, J.A
dc.contributor.authorHeath, W.R
dc.contributor.authorShortman, K
dc.date.accessioned2020-10-27T06:57:48Z
dc.date.available2020-10-27T06:57:48Z
dc.date.issued2007
dc.identifier.citationCaminschi, I, Ahmet, F, Heger, K, Brady, J, Nutt, S.L, Vremec, D, Pietersz, S, Lahoud, M.H, Schofield, L, Hansen, D.S, O'Keeffe, M, Smyth, M.J, Bedoui, S, Davey, G.M, Villadangos, J.A, Heath, W.R, Shortman, K (2007). Putative IKDCs are functionally and developmentally similar to natural killer cells, but not to dendritic cells. Journal of Experimental Medicine 204 (11) : 2579-2590. ScholarBank@NUS Repository. https://doi.org/10.1084/jem.20071351
dc.identifier.issn0022-1007
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/181030
dc.description.abstractInterferon-producing killer dendritic cells (IKDCs) have been described as possessing the lytic potential of NK cells and the antigen-presenting capacity of dendritic cells (DCs). In this study, we examine the lytic function and antigen-presenting capacity of mouse spleen IKDCs, including those found in DC preparations. IKDCs efficiently killed NK cell targets, without requiring additional activation stimuli. However, in our hands, when exposed to protein antigen or to MHC class II peptide, IKDCs induced little or no T cell proliferation relative to conventional DCs or plasmacytoid DCs, either before or after activation with CpG, or in several disease models. Certain developmental features indicated that IKDCs resembled NK cells more than DCs. IKDCs, like NK cells, did not express the transcription factor PU.1 and were absent from recombinase activating gene-2-null, common ?-chain-null (Rag2 -/-Il2rg-/-) mice. When cultured with IL-15 and -18, IKDCs proliferated extensively, like NK cells. Under these conditions, a proportion of expanded IKDCs and NK cells expressed high levels of surface MHC class II. However, even such MHC class II+ IKDCs and NK cells induced poor T cell proliferative responses compared with DCs. Thus, IKDCs resemble NK cells functionally, and neither cell type could be induced to be effective antigen-presenting cells. JEM © The Rockefeller University Press.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectantigen
dc.subjectinterferon
dc.subjectmajor histocompatibility antigen class 2
dc.subjectnatural killer cell receptor
dc.subjectRAG2 protein
dc.subjecttranscription factor PU 1
dc.subjectanimal cell
dc.subjectanimal model
dc.subjectantigen presenting cell
dc.subjectarticle
dc.subjectcell function
dc.subjectcell proliferation
dc.subjectcell stimulation
dc.subjectcell structure
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectdendritic cell
dc.subjectfemale
dc.subjectinterferon producing killer dendritic cell
dc.subjectmouse
dc.subjectnatural killer cell
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectT lymphocyte
dc.subjectAnimals
dc.subjectAntigens, CD45
dc.subjectDendritic Cells
dc.subjectHistocompatibility Antigens Class II
dc.subjectImmunophenotyping
dc.subjectIntegrin alpha2
dc.subjectInterferons
dc.subjectKiller Cells, Natural
dc.subjectLymphocyte Activation
dc.subjectMice
dc.subjectSpleen
dc.subjectT-Lymphocytes
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.1084/jem.20071351
dc.description.sourcetitleJournal of Experimental Medicine
dc.description.volume204
dc.description.issue11
dc.description.page2579-2590
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1084_jem_20071351.pdf4.04 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons