Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13287-015-0032-2
Title: Breast cancer resistance protein identifies clonogenic keratinocytes in human interfollicular epidermis
Authors: Ma, D
Chua, A.W.C
Yang, E
Teo, P
Ting, Y
Song, C
Lane, E.B 
Lee, S.T 
Keywords: alpha6 integrin
beta1 integrin
breast cancer resistance protein
cytokeratin 14
protein p63
ABC transporter
ABCG2 protein, human
alpha6 integrin
beta1 integrin
biological marker
breast cancer resistance protein
CKAP4 protein, human
cytokeratin 14
KRT14 protein, human
membrane protein
tumor protein
adult
animal experiment
Article
basement membrane
cell proliferation
clonogenesis
colony formation
controlled study
epidermis
flow cytometry
human
human cell
immunoblotting
immunofluorescence
in vitro study
in vivo study
keratinocyte
male
mouse
newborn
nonhuman
priority journal
protein expression
stem cell
animal
Bagg albino mouse
biosynthesis
cell culture
cell differentiation
cytology
epidermis
fluorescent antibody technique
keratinocyte
metabolism
nude mouse
physiology
skin
transplantation
xenograft
Animals
Antigens, CD29
ATP Binding Cassette Transporter, Sub-Family G, Member 2
ATP-Binding Cassette Transporters
Biomarkers
Cell Differentiation
Cells, Cultured
Epidermis
Fluorescent Antibody Technique
Humans
Immunoblotting
Integrin alpha6
Keratin-14
Keratinocytes
Membrane Proteins
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Proteins
Skin
Stem Cells
Transplantation, Heterologous
Issue Date: 2015
Citation: Ma, D, Chua, A.W.C, Yang, E, Teo, P, Ting, Y, Song, C, Lane, E.B, Lee, S.T (2015). Breast cancer resistance protein identifies clonogenic keratinocytes in human interfollicular epidermis. Stem Cell Research and Therapy 6 (1) : 32. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-015-0032-2
Rights: Attribution 4.0 International
Abstract: Abstract Introduction: There is a practical need for the identification of robust cell-surface markers that can be used to enrich for living keratinocyte progenitor cells. Breast cancer resistance protein (ABCG2), a member of the ATP binding cassette (ABC) transporter family, is known to be a marker for stem/progenitor cells in many tissues and organs. Methods: We investigated the expression of ABCG2 protein in normal human epidermis to evaluate its potential as a cell surface marker for identifying and enriching for clonogenic epidermal keratinocytes outside the pilosebaceous tract. Results: Immunofluorescence and immunoblotting studies of human skin showed that ABCG2 is expressed in a subset of basal layer cells in the epidermis. Flow cytometry analysis showed approximately 2-3% of keratinocytes in non-hair-bearing epidermis expressing ABCG2; this population also expresses p63, ?1 and ?6 integrins and keratin 14, but not CD34, CD71, C-kit or involucrin. The ABCG2-positive keratinocytes showed significantly higher colony forming efficiency when co-cultured with mouse 3T3 feeder cells, and more extensive long-term proliferation capacity in vitro, than did ABCG2-negative keratinocytes. Upon clonal analysis, most of the freshly isolated ABCG2-positive keratinocytes formed holoclones and were capable of generating a stratified differentiating epidermis in organotypic culture models. Conclusions: These data indicate that in skin, expression of the ABCG2 transporter is a characteristic of interfollicular keratinocyte progentior cells and suggest that ABCG2 may be useful for enriching keratinocyte stem cells in human interfollicular epidermis. © 2015 Ma et al.
Source Title: Stem Cell Research and Therapy
URI: https://scholarbank.nus.edu.sg/handle/10635/180876
ISSN: 17576512
DOI: 10.1186/s13287-015-0032-2
Rights: Attribution 4.0 International
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