Please use this identifier to cite or link to this item:
https://doi.org/10.1186/s13287-015-0032-2
DC Field | Value | |
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dc.title | Breast cancer resistance protein identifies clonogenic keratinocytes in human interfollicular epidermis | |
dc.contributor.author | Ma, D | |
dc.contributor.author | Chua, A.W.C | |
dc.contributor.author | Yang, E | |
dc.contributor.author | Teo, P | |
dc.contributor.author | Ting, Y | |
dc.contributor.author | Song, C | |
dc.contributor.author | Lane, E.B | |
dc.contributor.author | Lee, S.T | |
dc.date.accessioned | 2020-10-27T05:34:22Z | |
dc.date.available | 2020-10-27T05:34:22Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Ma, D, Chua, A.W.C, Yang, E, Teo, P, Ting, Y, Song, C, Lane, E.B, Lee, S.T (2015). Breast cancer resistance protein identifies clonogenic keratinocytes in human interfollicular epidermis. Stem Cell Research and Therapy 6 (1) : 32. ScholarBank@NUS Repository. https://doi.org/10.1186/s13287-015-0032-2 | |
dc.identifier.issn | 17576512 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/180876 | |
dc.description.abstract | Abstract Introduction: There is a practical need for the identification of robust cell-surface markers that can be used to enrich for living keratinocyte progenitor cells. Breast cancer resistance protein (ABCG2), a member of the ATP binding cassette (ABC) transporter family, is known to be a marker for stem/progenitor cells in many tissues and organs. Methods: We investigated the expression of ABCG2 protein in normal human epidermis to evaluate its potential as a cell surface marker for identifying and enriching for clonogenic epidermal keratinocytes outside the pilosebaceous tract. Results: Immunofluorescence and immunoblotting studies of human skin showed that ABCG2 is expressed in a subset of basal layer cells in the epidermis. Flow cytometry analysis showed approximately 2-3% of keratinocytes in non-hair-bearing epidermis expressing ABCG2; this population also expresses p63, ?1 and ?6 integrins and keratin 14, but not CD34, CD71, C-kit or involucrin. The ABCG2-positive keratinocytes showed significantly higher colony forming efficiency when co-cultured with mouse 3T3 feeder cells, and more extensive long-term proliferation capacity in vitro, than did ABCG2-negative keratinocytes. Upon clonal analysis, most of the freshly isolated ABCG2-positive keratinocytes formed holoclones and were capable of generating a stratified differentiating epidermis in organotypic culture models. Conclusions: These data indicate that in skin, expression of the ABCG2 transporter is a characteristic of interfollicular keratinocyte progentior cells and suggest that ABCG2 may be useful for enriching keratinocyte stem cells in human interfollicular epidermis. © 2015 Ma et al. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | alpha6 integrin | |
dc.subject | beta1 integrin | |
dc.subject | breast cancer resistance protein | |
dc.subject | cytokeratin 14 | |
dc.subject | protein p63 | |
dc.subject | ABC transporter | |
dc.subject | ABCG2 protein, human | |
dc.subject | alpha6 integrin | |
dc.subject | beta1 integrin | |
dc.subject | biological marker | |
dc.subject | breast cancer resistance protein | |
dc.subject | CKAP4 protein, human | |
dc.subject | cytokeratin 14 | |
dc.subject | KRT14 protein, human | |
dc.subject | membrane protein | |
dc.subject | tumor protein | |
dc.subject | adult | |
dc.subject | animal experiment | |
dc.subject | Article | |
dc.subject | basement membrane | |
dc.subject | cell proliferation | |
dc.subject | clonogenesis | |
dc.subject | colony formation | |
dc.subject | controlled study | |
dc.subject | epidermis | |
dc.subject | flow cytometry | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | immunoblotting | |
dc.subject | immunofluorescence | |
dc.subject | in vitro study | |
dc.subject | in vivo study | |
dc.subject | keratinocyte | |
dc.subject | male | |
dc.subject | mouse | |
dc.subject | newborn | |
dc.subject | nonhuman | |
dc.subject | priority journal | |
dc.subject | protein expression | |
dc.subject | stem cell | |
dc.subject | animal | |
dc.subject | Bagg albino mouse | |
dc.subject | biosynthesis | |
dc.subject | cell culture | |
dc.subject | cell differentiation | |
dc.subject | cytology | |
dc.subject | epidermis | |
dc.subject | fluorescent antibody technique | |
dc.subject | keratinocyte | |
dc.subject | metabolism | |
dc.subject | nude mouse | |
dc.subject | physiology | |
dc.subject | skin | |
dc.subject | transplantation | |
dc.subject | xenograft | |
dc.subject | Animals | |
dc.subject | Antigens, CD29 | |
dc.subject | ATP Binding Cassette Transporter, Sub-Family G, Member 2 | |
dc.subject | ATP-Binding Cassette Transporters | |
dc.subject | Biomarkers | |
dc.subject | Cell Differentiation | |
dc.subject | Cells, Cultured | |
dc.subject | Epidermis | |
dc.subject | Fluorescent Antibody Technique | |
dc.subject | Humans | |
dc.subject | Immunoblotting | |
dc.subject | Integrin alpha6 | |
dc.subject | Keratin-14 | |
dc.subject | Keratinocytes | |
dc.subject | Membrane Proteins | |
dc.subject | Mice | |
dc.subject | Mice, Inbred BALB C | |
dc.subject | Mice, Nude | |
dc.subject | Neoplasm Proteins | |
dc.subject | Skin | |
dc.subject | Stem Cells | |
dc.subject | Transplantation, Heterologous | |
dc.type | Article | |
dc.contributor.department | PATHOLOGY | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1186/s13287-015-0032-2 | |
dc.description.sourcetitle | Stem Cell Research and Therapy | |
dc.description.volume | 6 | |
dc.description.issue | 1 | |
dc.description.page | 32 | |
Appears in Collections: | Elements Staff Publications |
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