Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep14143
Title: MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - Comparison with human epileptic samples
Authors: Roncon, P
Soukupovà, M
Binaschi, A
Falcicchia, C
Zucchini, S
Ferracin, M
Langley, S.R 
Petretto, E
Johnson, M.R
Marucci, G
Michelucci, R
Rubboli, G
Simonato, M
Keywords: biological marker
microRNA
pilocarpine
transcriptome
adult
animal
blood
case control study
chemically induced
cluster analysis
cytology
epilepsy
female
gene expression profiling
gene expression regulation
gene regulatory network
genetics
hippocampus
human
male
metabolism
middle aged
pyramidal nerve cell
rat
Adult
Animals
Biomarkers
Case-Control Studies
Cluster Analysis
Epilepsy
Female
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Hippocampus
Humans
Male
MicroRNAs
Middle Aged
Pilocarpine
Pyramidal Cells
Rats
Transcriptome
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Roncon, P, Soukupovà, M, Binaschi, A, Falcicchia, C, Zucchini, S, Ferracin, M, Langley, S.R, Petretto, E, Johnson, M.R, Marucci, G, Michelucci, R, Rubboli, G, Simonato, M (2015). MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - Comparison with human epileptic samples. Scientific Reports 5 : 14143. ScholarBank@NUS Repository. https://doi.org/10.1038/srep14143
Rights: Attribution 4.0 International
Abstract: The identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury, and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis. © 2015, Nature Publishing Group. All rights reserved.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/180437
ISSN: 2045-2322
DOI: 10.1038/srep14143
Rights: Attribution 4.0 International
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