Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep14143
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dc.titleMicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - Comparison with human epileptic samples
dc.contributor.authorRoncon, P
dc.contributor.authorSoukupovà, M
dc.contributor.authorBinaschi, A
dc.contributor.authorFalcicchia, C
dc.contributor.authorZucchini, S
dc.contributor.authorFerracin, M
dc.contributor.authorLangley, S.R
dc.contributor.authorPetretto, E
dc.contributor.authorJohnson, M.R
dc.contributor.authorMarucci, G
dc.contributor.authorMichelucci, R
dc.contributor.authorRubboli, G
dc.contributor.authorSimonato, M
dc.date.accessioned2020-10-26T08:58:54Z
dc.date.available2020-10-26T08:58:54Z
dc.date.issued2015
dc.identifier.citationRoncon, P, Soukupovà, M, Binaschi, A, Falcicchia, C, Zucchini, S, Ferracin, M, Langley, S.R, Petretto, E, Johnson, M.R, Marucci, G, Michelucci, R, Rubboli, G, Simonato, M (2015). MicroRNA profiles in hippocampal granule cells and plasma of rats with pilocarpine-induced epilepsy - Comparison with human epileptic samples. Scientific Reports 5 : 14143. ScholarBank@NUS Repository. https://doi.org/10.1038/srep14143
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/180437
dc.description.abstractThe identification of biomarkers of the transformation of normal to epileptic tissue would help to stratify patients at risk of epilepsy following brain injury, and inform new treatment strategies. MicroRNAs (miRNAs) are an attractive option in this direction. In this study, miRNA microarrays were performed on laser-microdissected hippocampal granule cell layer (GCL) and on plasma, at different time points in the development of pilocarpine-induced epilepsy in the rat: latency, first spontaneous seizure and chronic epileptic phase. Sixty-three miRNAs were differentially expressed in the GCL when considering all time points. Three main clusters were identified that separated the control and chronic phase groups from the latency group and from the first spontaneous seizure group. MiRNAs from rats in the chronic phase were compared to those obtained from the laser-microdissected GCL of epileptic patients, identifying several miRNAs (miR-21-5p, miR-23a-5p, miR-146a-5p and miR-181c-5p) that were up-regulated in both human and rat epileptic tissue. Analysis of plasma samples revealed different levels between control and pilocarpine-treated animals for 27 miRNAs. Two main clusters were identified that segregated controls from all other groups. Those miRNAs that are altered in plasma before the first spontaneous seizure, like miR-9a-3p, may be proposed as putative biomarkers of epileptogenesis. © 2015, Nature Publishing Group. All rights reserved.
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectbiological marker
dc.subjectmicroRNA
dc.subjectpilocarpine
dc.subjecttranscriptome
dc.subjectadult
dc.subjectanimal
dc.subjectblood
dc.subjectcase control study
dc.subjectchemically induced
dc.subjectcluster analysis
dc.subjectcytology
dc.subjectepilepsy
dc.subjectfemale
dc.subjectgene expression profiling
dc.subjectgene expression regulation
dc.subjectgene regulatory network
dc.subjectgenetics
dc.subjecthippocampus
dc.subjecthuman
dc.subjectmale
dc.subjectmetabolism
dc.subjectmiddle aged
dc.subjectpyramidal nerve cell
dc.subjectrat
dc.subjectAdult
dc.subjectAnimals
dc.subjectBiomarkers
dc.subjectCase-Control Studies
dc.subjectCluster Analysis
dc.subjectEpilepsy
dc.subjectFemale
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation
dc.subjectGene Regulatory Networks
dc.subjectHippocampus
dc.subjectHumans
dc.subjectMale
dc.subjectMicroRNAs
dc.subjectMiddle Aged
dc.subjectPilocarpine
dc.subjectPyramidal Cells
dc.subjectRats
dc.subjectTranscriptome
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1038/srep14143
dc.description.sourcetitleScientific Reports
dc.description.volume5
dc.description.page14143
dc.published.statepublished
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