Please use this identifier to cite or link to this item: https://doi.org/10.1128/JCM.00975-17
Title: Evaluation of a novel reporter virus neutralization test for serological diagnosis of Zika and Dengue virus infection
Authors: Shan, C
Ortiz, D.A
Yang, Y
Wong, S.J
Kramer, L.D
Shi, P.-Y 
Loeffelholz, M.J
Ren, P
Keywords: immunoglobulin G antibody
immunoglobulin M antibody
neutralizing antibody
immunoglobulin M
neutralizing antibody
virus antibody
antibody titer
Article
controlled study
dengue
diagnostic accuracy
diagnostic test accuracy study
high risk patient
human
low risk patient
major clinical study
plaque reduction neutralization test
priority journal
reporter virus neutralization test
reproducibility
screening test
sensitivity and specificity
serodiagnosis
turnaround time
virus neutralization
Zika fever
animal
blood
cell line
Chlorocebus aethiops
dengue
Dengue virus
enzyme linked immunosorbent assay
immunology
procedures
serodiagnosis
Vero cell line
viremia
virology
Zika fever
Zika virus
Animals
Antibodies, Neutralizing
Antibodies, Viral
Cell Line
Cercopithecus aethiops
Dengue
Dengue Virus
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin M
Neutralization Tests
Reproducibility of Results
Sensitivity and Specificity
Vero Cells
Viremia
Zika Virus
Zika Virus Infection
Issue Date: 2017
Publisher: American Society for Microbiology
Citation: Shan, C, Ortiz, D.A, Yang, Y, Wong, S.J, Kramer, L.D, Shi, P.-Y, Loeffelholz, M.J, Ren, P (2017). Evaluation of a novel reporter virus neutralization test for serological diagnosis of Zika and Dengue virus infection. Journal of Clinical Microbiology 55 (10) : 3028-3036. ScholarBank@NUS Repository. https://doi.org/10.1128/JCM.00975-17
Rights: Attribution 4.0 International
Abstract: Currently, the laboratory diagnosis of Zika virus (ZIKV) infection is primarily through the detection of ZIKV RNA or antibodies against ZIKV proteins. The detection of viral RNA is highly sensitive and specific, but periods of viremia and viruria are brief, limiting the utility of ZIKV RNA assays. Instead, most ZIKV infections are diagnosed serologically, using an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for screening, followed by a confirmatory plaque reduction neutralization test (PRNT). Typical turnaround times vary, due to assay incubation periods and a lack of clinical laboratories performing these tests. Recently, a novel luciferase-ZIKV-and-dengue virus (DENV)-based serological assay, which considerably improves the turnaround times and throughput for ZIKV diagnosis, was described. Using the traditional PRNT as a reference method, we evaluated the performance characteristics of the reporter virus neutralization test (RVNT) with 258 clinical serum specimens. The ZIKV RVNT produced primary ZIKV screening and secondary confirmation results in 4 days, with 100% reproducibility. As a screening assay, the ZIKV RVNT displayed excellent diagnostic accuracy, sensitivity, and specificity of 98.2%, 100%, and 98.1%, respectively. As a confirmatory assay, the ZIKV RVNT titers displayed 93.1% agreement with the traditional ZIKV PRNT titers. Overall, the RVNT accurately and reliably detects neutralizing antibodies in patient serum specimens, with improved turnaround times, and can be used for the serological detection of ZIKV infections. Due to the homogeneous 96-well format, the RVNT has also significantly improved the assay throughput to allow testing of a large number of specimens in a single run. © 2017 Shan et al.
Source Title: Journal of Clinical Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/179088
ISSN: 00951137
DOI: 10.1128/JCM.00975-17
Rights: Attribution 4.0 International
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