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Title: Theoretical methods for studying DNA structural transitions under applied mechanical constraints
Authors: Efremov, A.K 
Winardhi, R.S 
Yan, J 
Keywords: Biopolymers
Mechanical stability
Nucleic acids
Phase transitions
DNA-binding protein
Equilibrium thermodynamics
Force spectroscopy
Mechanical constraints
Single molecule experiments
Single molecule manipulation
Structural transitions
Theoretical framework
Issue Date: 2017
Citation: Efremov, A.K, Winardhi, R.S, Yan, J (2017). Theoretical methods for studying DNA structural transitions under applied mechanical constraints. Polymers 9 (2) : 74. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Recent progress in single-molecule manipulation technologies has made it possible to exert force and torque on individual DNA biopolymers to probe their mechanical stability and interaction with various DNA-binding proteins. It was revealed in these experiments that the DNA structure and formation of nucleoprotein complexes by DNA-architectural proteins can be strongly modulated by an intricate interplay between the entropic elasticity of DNA and its global topology, which is closely related to the mechanical constraints applied to the DNA. Detailed understanding of the physical processes underlying the DNA behavior observed in single-molecule experiments requires the development of a general theoretical framework, which turned out to be a rather challenging task. Here, we review recent advances in theoretical methods that can be used to interpret single-molecule manipulation experiments on DNA. © 2017 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: Polymers
ISSN: 20734360
DOI: 10.3390/polym9020074
Rights: Attribution 4.0 International
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