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https://doi.org/10.1038/s41467-017-00737-8
| Title: | A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage | Authors: | Shan, C Muruato, A.E Jagger, B.W Richner, J Nunes, B.T.D Medeiros, D.B.A Xie, X Nunes, J.G.C Morabito, K.M Kong, W.-P Pierson, T.C Barrett, A.D Weaver, S.C Rossi, S.L Vasconcelos, P.F.C Graham, B.S Diamond, M.S Shi, P.-Y |
Keywords: | live vaccine neutralizing antibody virus RNA Zika virus vaccine live vaccine virus vaccine abnormality disease transmission embryo embryonic development genome immunization infectious disease male nervous system disorder pregnancy primate reproductive health rodent sexually transmitted disease vaccine virus Zika virus disease 3' untranslated region adult animal experiment animal model animal tissue antibody blood level antibody response antibody titer Article comparative study controlled study embryo female fetus fetus tissue focus forming assay gene deletion limit of detection male maternal blood mouse nonhuman oligospermia orchitis placenta tissue pregnancy rhesus monkey single drug dose spermatozoon count spermatozoon motility testis injury vaccination vaccine immunogenicity vertical transmission virus genome virus load virus replication virus transmission Zika fever animal C57BL mouse genetics immunology pathology preclinical study pregnancy prevention and control testis transmission vertical transmission virology Zika fever Zika virus Human immunodeficiency virus 1 Macaca mulatta Mus Primates Zika virus Animals Drug Evaluation, Preclinical Female Infectious Disease Transmission, Vertical Male Mice Mice, Inbred C57BL Pregnancy Testis Vaccines, Attenuated Viral Vaccines Zika Virus Zika Virus Infection |
Issue Date: | 2017 | Publisher: | Nature Publishing Group | Citation: | Shan, C, Muruato, A.E, Jagger, B.W, Richner, J, Nunes, B.T.D, Medeiros, D.B.A, Xie, X, Nunes, J.G.C, Morabito, K.M, Kong, W.-P, Pierson, T.C, Barrett, A.D, Weaver, S.C, Rossi, S.L, Vasconcelos, P.F.C, Graham, B.S, Diamond, M.S, Shi, P.-Y (2017). A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage. Nature Communications 8 (1) : 676. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00737-8 | Rights: | Attribution 4.0 International | Abstract: | Zika virus infection during pregnancy can cause congenital abnormities or fetal demise. The persistence of Zika virus in the male reproductive system poses a risk of sexual transmission. Here we demonstrate that live-attenuated Zika virus vaccine candidates containing deletions in the 3? untranslated region of the Zika virus genome (ZIKV-3?UTR-LAV) prevent viral transmission during pregnancy and testis damage in mice, as well as infection of nonhuman primates. After a single-dose vaccination, pregnant mice challenged with Zika virus at embryonic day 6 and evaluated at embryonic day 13 show markedly diminished levels of viral RNA in maternal, placental, and fetal tissues. Vaccinated male mice challenged with Zika virus were protected against testis infection, injury, and oligospermia. A single immunization of rhesus macaques elicited a rapid and robust antibody response, conferring complete protection upon challenge. Furthermore, the ZIKV-3?UTR-LAV vaccine candidates have a desirable safety profile. These results suggest that further development of ZIKV-3?UTR-LAV is warranted for humans. © 2017 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/178574 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-017-00737-8 | Rights: | Attribution 4.0 International |
| Appears in Collections: | Staff Publications Elements |
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