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https://doi.org/10.1038/s41467-018-02975-w
| Title: | Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone | Authors: | Li, X.-F Dong, H.-L Wang, H.-J Huang, X.-Y Qiu, Y.-F Ji, X Ye, Q Li, C Liu, Y Deng, Y.-Q Jiang, T Cheng, G Zhang, F.-C Davidson, A.D Song, Y.-J Shi, P.-Y Qin, C.-F |
Keywords: | epidermal growth factor receptor gamma interferon gamma interferon inducible protein 10 granulocyte macrophage colony stimulating factor immunoglobulin G interleukin 10 interleukin 15 interleukin 1beta interleukin 2 interleukin 8 Japanese encephalitis vaccine live vaccine macrophage inflammatory protein 1beta macrophage migration inhibition factor monocyte chemotactic protein 1 neutralizing antibody RANTES sa 14 14 2 virus RNA Zika virus vaccine live vaccine neutralizing antibody recombinant vaccine virus antibody virus vaccine encephalitis gene expression genetic analysis immune response immunization phenotype protein vaccine Zika virus disease animal experiment animal model animal tissue antibody blood level Article cellular immunity controlled study cytokine production drug efficacy female genetic stability in vitro study in vivo study infection prevention male mouse nonhuman protein blood level rhesus monkey single drug dose vaccination vaccine immunogenicity virus attenuation Zika fever 129 mouse Aedes animal Bagg albino mouse biosynthesis disease model genetic engineering genetics human immunology Japanese encephalitis virus mosquito vector pregnancy prevention and control vertical transmission viremia virology Zika fever Zika virus Animalia Flavivirus Japanese encephalitis virus Macaca mulatta Mus Zika virus Aedes Animals Antibodies, Neutralizing Antibodies, Viral Disease Models, Animal Encephalitis Virus, Japanese Female Genetic Engineering Humans Infectious Disease Transmission, Vertical Macaca mulatta Male Mice Mice, 129 Strain Mice, Inbred BALB C Mosquito Vectors Pregnancy Vaccines, Attenuated Vaccines, Synthetic Viral Vaccines Viremia Zika Virus Zika Virus Infection |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Li, X.-F, Dong, H.-L, Wang, H.-J, Huang, X.-Y, Qiu, Y.-F, Ji, X, Ye, Q, Li, C, Liu, Y, Deng, Y.-Q, Jiang, T, Cheng, G, Zhang, F.-C, Davidson, A.D, Song, Y.-J, Shi, P.-Y, Qin, C.-F (2018). Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nature Communications 9 (1) : 673. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-02975-w | Rights: | Attribution 4.0 International | Abstract: | The global spread of Zika virus (ZIKV) and its unexpected association with congenital defects necessitates the rapid development of a safe and effective vaccine. Here we report the development and characterization of a recombinant chimeric ZIKV vaccine candidate (termed ChinZIKV) that expresses the prM-E proteins of ZIKV using the licensed Japanese encephalitis live-attenuated vaccine SA14-14-2 as the genetic backbone. ChinZIKV retains its replication activity and genetic stability in vitro, while exhibiting an attenuation phenotype in multiple animal models. Remarkably, immunization of mice and rhesus macaques with a single dose of ChinZIKV elicits robust and long-lasting immune responses, and confers complete protection against ZIKV challenge. Significantly, female mice immunized with ChinZIKV are protected against placental and fetal damage upon ZIKV challenge during pregnancy. Overall, our study provides an alternative vaccine platform in response to the ZIKV emergency, and the safety, immunogenicity, and protection profiles of ChinZIKV warrant further clinical development. © 2018 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/178453 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-018-02975-w | Rights: | Attribution 4.0 International |
| Appears in Collections: | Staff Publications Elements |
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