Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-06077-3
Title: Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
Authors: Reid, D.W
Xu, D 
Chen, P
Yang, H
Sun, L 
Keywords: messenger RNA
microRNA
transcriptome
adipogenesis
animal
brown adipocyte
brown adipose tissue
C57BL mouse
genetics
male
metabolism
mitochondrion
ribosome
white adipocyte
white adipose tissue
Adipocytes, Brown
Adipocytes, White
Adipogenesis
Adipose Tissue, Brown
Adipose Tissue, White
Animals
Male
Mice, Inbred C57BL
MicroRNAs
Mitochondria
Ribosomes
RNA, Messenger
Transcriptome
Issue Date: 2017
Citation: Reid, D.W, Xu, D, Chen, P, Yang, H, Sun, L (2017). Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs. Scientific Reports 7 (1) : 5681. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-06077-3
Rights: Attribution 4.0 International
Abstract: The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets' mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178313
ISSN: 20452322
DOI: 10.1038/s41598-017-06077-3
Rights: Attribution 4.0 International
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