Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-06077-3
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dc.titleIntegrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
dc.contributor.authorReid, D.W
dc.contributor.authorXu, D
dc.contributor.authorChen, P
dc.contributor.authorYang, H
dc.contributor.authorSun, L
dc.date.accessioned2020-10-20T09:10:31Z
dc.date.available2020-10-20T09:10:31Z
dc.date.issued2017
dc.identifier.citationReid, D.W, Xu, D, Chen, P, Yang, H, Sun, L (2017). Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs. Scientific Reports 7 (1) : 5681. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-06077-3
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/178313
dc.description.abstractThe epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets' mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue. © 2017 The Author(s).
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectmessenger RNA
dc.subjectmicroRNA
dc.subjecttranscriptome
dc.subjectadipogenesis
dc.subjectanimal
dc.subjectbrown adipocyte
dc.subjectbrown adipose tissue
dc.subjectC57BL mouse
dc.subjectgenetics
dc.subjectmale
dc.subjectmetabolism
dc.subjectmitochondrion
dc.subjectribosome
dc.subjectwhite adipocyte
dc.subjectwhite adipose tissue
dc.subjectAdipocytes, Brown
dc.subjectAdipocytes, White
dc.subjectAdipogenesis
dc.subjectAdipose Tissue, Brown
dc.subjectAdipose Tissue, White
dc.subjectAnimals
dc.subjectMale
dc.subjectMice, Inbred C57BL
dc.subjectMicroRNAs
dc.subjectMitochondria
dc.subjectRibosomes
dc.subjectRNA, Messenger
dc.subjectTranscriptome
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1038/s41598-017-06077-3
dc.description.sourcetitleScientific Reports
dc.description.volume7
dc.description.issue1
dc.description.page5681
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