Please use this identifier to cite or link to this item: https://doi.org/10.1038/msb.2011.55
Title: Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen
Authors: Saeidi, N 
Wong, C.K
Lo, T.-M 
Nguyen, H.X
Ling, H 
Leong, S.S.J 
Poh, C.L 
Chang, M.W 
Keywords: pyocin
antiinfective agent
bacterial protein
drug derivative
gamma butyrolactone
green fluorescent protein
homoserine
hybrid protein
n (3 oxododecanoyl)homoserine lactone
N-(3-oxododecanoyl)homoserine lactone
pyocin
article
bacterial strain
cell viability
controlled study
Escherichia coli
genetic engineering and gene technology
nonhuman
priority journal
protein secretion
Pseudomonas aeruginosa
quorum sensing
antibiosis
biofilm
biosynthesis
chemistry
drug effect
Escherichia coli
gene expression regulation
genetic procedures
genetics
growth, development and aging
human
metabolism
methodology
microbiology
pathogenicity
plasmid
Pseudomonas infection
quorum sensing
reporter gene
synthetic biology
transgenic organism
Escherichia coli
Pseudomonas aeruginosa
Escherichia coli
Pseudomonas aeruginosa
4-Butyrolactone
Anti-Bacterial Agents
Antibiosis
Bacterial Proteins
Biofilms
Biosensing Techniques
Escherichia coli
Gene Expression Regulation, Bacterial
Genes, Reporter
Green Fluorescent Proteins
Homoserine
Humans
Organisms, Genetically Modified
Plasmids
Pseudomonas aeruginosa
Pseudomonas Infections
Pyocins
Quorum Sensing
Recombinant Fusion Proteins
Synthetic Biology
Issue Date: 2011
Publisher: EMBO Press
Citation: Saeidi, N, Wong, C.K, Lo, T.-M, Nguyen, H.X, Ling, H, Leong, S.S.J, Poh, C.L, Chang, M.W (2011). Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Molecular Systems Biology 7 : 521. ScholarBank@NUS Repository. https://doi.org/10.1038/msb.2011.55
Rights: Attribution 4.0 International
Abstract: Synthetic biology aims to systematically design and construct novel biological systems that address energy, environment, and health issues. Herein, we describe the development of a synthetic genetic system, which comprises quorum sensing, killing, and lysing devices, that enables Escherichia coli to sense and kill a pathogenic Pseudomonas aeruginosa strain through the production and release of pyocin. The sensing, killing, and lysing devices were characterized to elucidate their detection, antimicrobial and pyocin release functionalities, which subsequently aided in the construction of the final system and the verification of its designed behavior. We demonstrated that our engineered E. coli sensed and killed planktonic P. aeruginosa, evidenced by 99% reduction in the viable cells. Moreover, we showed that our engineered E. coli inhibited the formation of P. aeruginosa biofilm by close to 90%, leading to much sparser and thinner biofilm matrices. These results suggest that E. coli carrying our synthetic genetic system may provide a novel synthetic biology-driven antimicrobial strategy that could potentially be applied to fighting P. aeruginosa and other infectious pathogens. © 2011 EMBO and Macmillan Publishers Limited.
Source Title: Molecular Systems Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/178171
ISSN: 1744-4292
DOI: 10.1038/msb.2011.55
Rights: Attribution 4.0 International
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