Please use this identifier to cite or link to this item: https://doi.org/10.3389/fimmu.2018.02655
Title: Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
Authors: Castro-Sánchez, P
Ramirez-Munoz, R
Martín-Cófreces, N.B
Aguilar-Sopeña, O
Alegre-Gomez, S
Hernández-Pérez, S
Reyes, R
Zeng, Q 
Cabañas, C
Sánchez-Madrid, F
Roda-Navarro, P
Keywords: actin
interleukin 2
ionomycin
peptides and proteins
pericentriolar material 1
phosphatase of regenerating liver 1
phosphatase of regenerating liver 2
phosphatase of regenerating liver 3
procyanidin B3
PTP4A1 protein
PTP4A2 protein
PTP4A3 protein
unclassified drug
actin
CD3 antigen
CD3E protein, human
cell cycle protein
IL2 protein, human
interleukin 2
lymphocyte function associated antigen 1
membrane protein
protein tyrosine phosphatase
PTP4A1 protein, human
Article
CD4+ T lymphocyte
cell function
confocal microscopy
controlled study
down regulation
enzyme activity
enzyme linked immunosorbent assay
gene control
gene expression
genetic transfection
human
human cell
immune response
immunofluorescence
immunological synapse
mRNA expression level
plasmid
protein expression
Western blotting
female
immunological synapse
immunology
lymphocyte activation
male
T lymphocyte
Actins
CD3 Complex
Cell Cycle Proteins
Female
Humans
Immunological Synapses
Interleukin-2
Lymphocyte Activation
Lymphocyte Function-Associated Antigen-1
Male
Membrane Proteins
Protein Tyrosine Phosphatases
T-Lymphocytes
Issue Date: 2018
Citation: Castro-Sánchez, P, Ramirez-Munoz, R, Martín-Cófreces, N.B, Aguilar-Sopeña, O, Alegre-Gomez, S, Hernández-Pérez, S, Reyes, R, Zeng, Q, Cabañas, C, Sánchez-Madrid, F, Roda-Navarro, P (2018). Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function. Frontiers in Immunology 9 (NOV) : 2655. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2018.02655
Rights: Attribution 4.0 International
Abstract: The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunological Synapse (IS). We found that T cell activation downregulates the expression of PRL-2, resulting in an increased PRL-1/PRL-2 ratio. PRL-1 redistributed at the IS in two stages: Initially, it was transiently accumulated at scanning membranes enriched in CD3 and actin, and at later times, it was delivered at the contact site from pericentriolar, CD3?-containing, vesicles. Once at the established IS, PRL-1 distributed to LFA-1 and CD3? sites. Remarkably, PRL-1 was found to regulate actin dynamics during IS assembly and the secretion of IL-2. Moreover, pharmacological inhibition of the catalytic activity of the three PRLs reduced the secretion of IL-2. These results provide evidence indicating a regulatory role of PRL-1 during IS assembly and highlight the involvement of PRLs in immune responses by mature T cells. © 2007 - 2018 Frontiers Media S.A.
Source Title: Frontiers in Immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/178058
ISSN: 16643224
DOI: 10.3389/fimmu.2018.02655
Rights: Attribution 4.0 International
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