Please use this identifier to cite or link to this item:
https://doi.org/10.3389/fimmu.2018.02655
DC Field | Value | |
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dc.title | Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function | |
dc.contributor.author | Castro-Sánchez, P | |
dc.contributor.author | Ramirez-Munoz, R | |
dc.contributor.author | Martín-Cófreces, N.B | |
dc.contributor.author | Aguilar-Sopeña, O | |
dc.contributor.author | Alegre-Gomez, S | |
dc.contributor.author | Hernández-Pérez, S | |
dc.contributor.author | Reyes, R | |
dc.contributor.author | Zeng, Q | |
dc.contributor.author | Cabañas, C | |
dc.contributor.author | Sánchez-Madrid, F | |
dc.contributor.author | Roda-Navarro, P | |
dc.date.accessioned | 2020-10-20T04:58:03Z | |
dc.date.available | 2020-10-20T04:58:03Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Castro-Sánchez, P, Ramirez-Munoz, R, Martín-Cófreces, N.B, Aguilar-Sopeña, O, Alegre-Gomez, S, Hernández-Pérez, S, Reyes, R, Zeng, Q, Cabañas, C, Sánchez-Madrid, F, Roda-Navarro, P (2018). Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function. Frontiers in Immunology 9 (NOV) : 2655. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2018.02655 | |
dc.identifier.issn | 16643224 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/178058 | |
dc.description.abstract | The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunological Synapse (IS). We found that T cell activation downregulates the expression of PRL-2, resulting in an increased PRL-1/PRL-2 ratio. PRL-1 redistributed at the IS in two stages: Initially, it was transiently accumulated at scanning membranes enriched in CD3 and actin, and at later times, it was delivered at the contact site from pericentriolar, CD3?-containing, vesicles. Once at the established IS, PRL-1 distributed to LFA-1 and CD3? sites. Remarkably, PRL-1 was found to regulate actin dynamics during IS assembly and the secretion of IL-2. Moreover, pharmacological inhibition of the catalytic activity of the three PRLs reduced the secretion of IL-2. These results provide evidence indicating a regulatory role of PRL-1 during IS assembly and highlight the involvement of PRLs in immune responses by mature T cells. © 2007 - 2018 Frontiers Media S.A. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | actin | |
dc.subject | interleukin 2 | |
dc.subject | ionomycin | |
dc.subject | peptides and proteins | |
dc.subject | pericentriolar material 1 | |
dc.subject | phosphatase of regenerating liver 1 | |
dc.subject | phosphatase of regenerating liver 2 | |
dc.subject | phosphatase of regenerating liver 3 | |
dc.subject | procyanidin B3 | |
dc.subject | PTP4A1 protein | |
dc.subject | PTP4A2 protein | |
dc.subject | PTP4A3 protein | |
dc.subject | unclassified drug | |
dc.subject | actin | |
dc.subject | CD3 antigen | |
dc.subject | CD3E protein, human | |
dc.subject | cell cycle protein | |
dc.subject | IL2 protein, human | |
dc.subject | interleukin 2 | |
dc.subject | lymphocyte function associated antigen 1 | |
dc.subject | membrane protein | |
dc.subject | protein tyrosine phosphatase | |
dc.subject | PTP4A1 protein, human | |
dc.subject | Article | |
dc.subject | CD4+ T lymphocyte | |
dc.subject | cell function | |
dc.subject | confocal microscopy | |
dc.subject | controlled study | |
dc.subject | down regulation | |
dc.subject | enzyme activity | |
dc.subject | enzyme linked immunosorbent assay | |
dc.subject | gene control | |
dc.subject | gene expression | |
dc.subject | genetic transfection | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | immune response | |
dc.subject | immunofluorescence | |
dc.subject | immunological synapse | |
dc.subject | mRNA expression level | |
dc.subject | plasmid | |
dc.subject | protein expression | |
dc.subject | Western blotting | |
dc.subject | female | |
dc.subject | immunological synapse | |
dc.subject | immunology | |
dc.subject | lymphocyte activation | |
dc.subject | male | |
dc.subject | T lymphocyte | |
dc.subject | Actins | |
dc.subject | CD3 Complex | |
dc.subject | Cell Cycle Proteins | |
dc.subject | Female | |
dc.subject | Humans | |
dc.subject | Immunological Synapses | |
dc.subject | Interleukin-2 | |
dc.subject | Lymphocyte Activation | |
dc.subject | Lymphocyte Function-Associated Antigen-1 | |
dc.subject | Male | |
dc.subject | Membrane Proteins | |
dc.subject | Protein Tyrosine Phosphatases | |
dc.subject | T-Lymphocytes | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.3389/fimmu.2018.02655 | |
dc.description.sourcetitle | Frontiers in Immunology | |
dc.description.volume | 9 | |
dc.description.issue | NOV | |
dc.description.page | 2655 | |
Appears in Collections: | Staff Publications Elements |
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