Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2018.02656
Title: A new role of OmpR in acid and osmotic stress in salmonella and E. coli
Authors: Chakraborty, S 
Kenney, L.J 
Keywords: outer membrane protein regulator
RNA 16S
sigma factor RpoS
acidification
acidity
Article
atomic force microscopy
bacterial growth
bacterial metabolism
bacterial virulence
controlled study
Escherichia coli
gene ontology
gene overexpression
microarray analysis
nonhuman
osmolality
osmotic stress
pH
protein expression
real time polymerase chain reaction
regulon
reverse transcription polymerase chain reaction
RNA isolation
Salmonella enterica serovar Typhimurium
signal transduction
transcription regulation
Issue Date: 2018
Citation: Chakraborty, S, Kenney, L.J (2018). A new role of OmpR in acid and osmotic stress in salmonella and E. coli. Frontiers in Microbiology 9 (NOV) : 2656. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2018.02656
Rights: Attribution 4.0 International
Abstract: Bacteria survive and respond to diverse environmental conditions and during infection inside the host by systematic regulation of stress response genes. E. coli and S. Typhimurium can undergo large changes in intracellular osmolality (up to 1.8 Osmol/kg) and can tolerate cytoplasmic acidification to at least pHi 5.6. Recent analyses of single cells challenged a long held view that bacteria respond to extracellular acid stress by rapid acidification followed by a rapid recovery. It is now appreciated that both S. Typhimurium and E. coli maintain an acidic cytoplasm through the actions of the outer membrane protein regulator OmpR via its regulation of distinct signaling pathways. However, a comprehensive comparison of OmpR regulons between S. Typhimurium and E. coli is lacking. In this study, we examined the expression profiles of wild-type and ompR null strains of the intracellular pathogen S. Typhimurium and a commensal E. coli in response to acid and osmotic stress. Herein, we classify distinct OmpR regulons and also identify shared OmpR regulatory pathways between S. Typhimurium and E. coli in response to acid and osmotic stress. Our study establishes OmpR as a key regulator of bacterial virulence, growth and metabolism, in addition to its role in regulating outer membrane proteins. © 2018 Chakraborty and Kenney.
Source Title: Frontiers in Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/178057
ISSN: 1664302X
DOI: 10.3389/fmicb.2018.02656
Rights: Attribution 4.0 International
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