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https://doi.org/10.1186/1471-213X-7-99
Title: | Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY | Authors: | Herpin, A Schindler, D Kraiss, A Hornung, U Winkler, C Schartl, M |
Keywords: | article cell cycle progression cell proliferation Dmrt1 gene down regulation flow cytometry gene expression germ cell gonad development mitosis inhibition nonhuman Oryzias Precambrian regulator gene Sertoli cell sex determination sex differentiation animal animal embryo cell culture cell nucleus cell proliferation cytology DNA replication female gender and sex gene expression regulation genetics germ cell gonad in vitro study male metabolism Oryzias phenotype prenatal development time transgenic animal Mammalia Oryziinae Vertebrata DMRT1 protein DNA transcription factor unclassified drug Animals Animals, Genetically Modified Cell Nucleus Cell Proliferation Cells, Cultured DNA DNA Replication Down-Regulation Embryo, Nonmammalian Female Gene Expression Regulation, Developmental Germ Cells Gonads Male Oryzias Phenotype Sex Determination (Genetics) Time Factors Transcription Factors |
Issue Date: | 2007 | Citation: | Herpin, A, Schindler, D, Kraiss, A, Hornung, U, Winkler, C, Schartl, M (2007). Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY. BMC Developmental Biology 7 : 99. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-213X-7-99 | Rights: | Attribution 4.0 International | Abstract: | Background. Dmrt1 is a highly conserved gene involved in the determination and early differentiation phase of the primordial gonad in vertebrates. In the fish medaka dmrt1bY, a functional duplicate of the autosomal dmrt1a gene on the Y-chromosome, has been shown to be the master regulator of male gonadal development, comparable to Sry in mammals. In males mRNA and protein expression was observed before morphological sex differentiation in the somatic cells surrounding primordial germ cells (PGCs) of the gonadal anlage and later on exclusively in Sertoli cells. This suggested a role for dmrt1bY during male gonad and germ cell development. Results. We provide functional evidence that expression of dmrt1bY leads to negative regulation of PGC proliferation. Flow cytometric measurements revealed a G2 arrest of dmrt1bY expressing cells. Interestingly, also non-transfected cells displayed a significantly lower fraction of proliferating cells, pointing to a possible non-cell autonomous action of dmrt1bY. Injection of antisense morpholinos led to an increase of PGCs in genetically male embryos due to loss of proliferation inhibition. Conclusion. In medaka, dmrt1bY mediates a mitotic arrest of PGCs in males prior to testes differentiation at the sex determination stage. This occurs possibly via a cross-talk of Sertoli cells and PGCs. © 2007 Herpin et al; licensee BioMed Central Ltd. | Source Title: | BMC Developmental Biology | URI: | https://scholarbank.nus.edu.sg/handle/10635/177987 | ISSN: | 1471213X | DOI: | 10.1186/1471-213X-7-99 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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