Please use this identifier to cite or link to this item:
https://doi.org/10.1186/1471-213X-7-99
DC Field | Value | |
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dc.title | Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY | |
dc.contributor.author | Herpin, A | |
dc.contributor.author | Schindler, D | |
dc.contributor.author | Kraiss, A | |
dc.contributor.author | Hornung, U | |
dc.contributor.author | Winkler, C | |
dc.contributor.author | Schartl, M | |
dc.date.accessioned | 2020-10-20T04:45:14Z | |
dc.date.available | 2020-10-20T04:45:14Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Herpin, A, Schindler, D, Kraiss, A, Hornung, U, Winkler, C, Schartl, M (2007). Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY. BMC Developmental Biology 7 : 99. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-213X-7-99 | |
dc.identifier.issn | 1471213X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/177987 | |
dc.description.abstract | Background. Dmrt1 is a highly conserved gene involved in the determination and early differentiation phase of the primordial gonad in vertebrates. In the fish medaka dmrt1bY, a functional duplicate of the autosomal dmrt1a gene on the Y-chromosome, has been shown to be the master regulator of male gonadal development, comparable to Sry in mammals. In males mRNA and protein expression was observed before morphological sex differentiation in the somatic cells surrounding primordial germ cells (PGCs) of the gonadal anlage and later on exclusively in Sertoli cells. This suggested a role for dmrt1bY during male gonad and germ cell development. Results. We provide functional evidence that expression of dmrt1bY leads to negative regulation of PGC proliferation. Flow cytometric measurements revealed a G2 arrest of dmrt1bY expressing cells. Interestingly, also non-transfected cells displayed a significantly lower fraction of proliferating cells, pointing to a possible non-cell autonomous action of dmrt1bY. Injection of antisense morpholinos led to an increase of PGCs in genetically male embryos due to loss of proliferation inhibition. Conclusion. In medaka, dmrt1bY mediates a mitotic arrest of PGCs in males prior to testes differentiation at the sex determination stage. This occurs possibly via a cross-talk of Sertoli cells and PGCs. © 2007 Herpin et al; licensee BioMed Central Ltd. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | article | |
dc.subject | cell cycle progression | |
dc.subject | cell proliferation | |
dc.subject | Dmrt1 gene | |
dc.subject | down regulation | |
dc.subject | flow cytometry | |
dc.subject | gene expression | |
dc.subject | germ cell | |
dc.subject | gonad development | |
dc.subject | mitosis inhibition | |
dc.subject | nonhuman | |
dc.subject | Oryzias | |
dc.subject | Precambrian | |
dc.subject | regulator gene | |
dc.subject | Sertoli cell | |
dc.subject | sex determination | |
dc.subject | sex differentiation | |
dc.subject | animal | |
dc.subject | animal embryo | |
dc.subject | cell culture | |
dc.subject | cell nucleus | |
dc.subject | cell proliferation | |
dc.subject | cytology | |
dc.subject | DNA replication | |
dc.subject | female | |
dc.subject | gender and sex | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | germ cell | |
dc.subject | gonad | |
dc.subject | in vitro study | |
dc.subject | male | |
dc.subject | metabolism | |
dc.subject | Oryzias | |
dc.subject | phenotype | |
dc.subject | prenatal development | |
dc.subject | time | |
dc.subject | transgenic animal | |
dc.subject | Mammalia | |
dc.subject | Oryziinae | |
dc.subject | Vertebrata | |
dc.subject | DMRT1 protein | |
dc.subject | DNA | |
dc.subject | transcription factor | |
dc.subject | unclassified drug | |
dc.subject | Animals | |
dc.subject | Animals, Genetically Modified | |
dc.subject | Cell Nucleus | |
dc.subject | Cell Proliferation | |
dc.subject | Cells, Cultured | |
dc.subject | DNA | |
dc.subject | DNA Replication | |
dc.subject | Down-Regulation | |
dc.subject | Embryo, Nonmammalian | |
dc.subject | Female | |
dc.subject | Gene Expression Regulation, Developmental | |
dc.subject | Germ Cells | |
dc.subject | Gonads | |
dc.subject | Male | |
dc.subject | Oryzias | |
dc.subject | Phenotype | |
dc.subject | Sex Determination (Genetics) | |
dc.subject | Time Factors | |
dc.subject | Transcription Factors | |
dc.type | Article | |
dc.contributor.department | BIOLOGY (NU) | |
dc.description.doi | 10.1186/1471-213X-7-99 | |
dc.description.sourcetitle | BMC Developmental Biology | |
dc.description.volume | 7 | |
dc.description.page | 99 | |
Appears in Collections: | Staff Publications Elements |
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