Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep18146
Title: Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF
Authors: Lee, S.Y 
Lim, C.J 
Dr”ge, P
Yan, J 
Keywords: bacterial DNA
DNA binding protein
Escherichia coli protein
integration host factor
magnesium chloride
potassium chloride
protein binding
bacterial genome
binding competition
DNA packaging
drug effects
Escherichia coli
gene expression regulation
genetics
growth, development and aging
magnetism
metabolism
optical tweezers
pH
Binding, Competitive
DNA Packaging
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Genome, Bacterial
Hydrogen-Ion Concentration
Integration Host Factors
Magnesium Chloride
Magnetics
Optical Tweezers
Potassium Chloride
Protein Binding
Issue Date: 2015
Citation: Lee, S.Y, Lim, C.J, Dr”ge, P, Yan, J (2015). Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF. Scientific Reports 5 : 18146. ScholarBank@NUS Repository. https://doi.org/10.1038/srep18146
Abstract: The bacterial nucleoid, a bacterial genome packed by nucleoid binding proteins, forms the physical basis for cellular processes such as gene transcription and DNA replication. Bacteria need to dynamically modulate their nucleoid structures at different growth phases and in response to environmental changes. At the nutrients deficient stationary phase, DNA-binding proteins from starved cells (Dps) and Integration host factors (IHF) are the two most abundant nucleoid associated proteins in E. coli. Yet, it remains unclear how the nucleoid architecture is controlled by the interplay between these two proteins, as well as the nucleoid's response to environmental changes. This question is addressed here using single DNA manipulation approach. Our results reveal that the two proteins are differentially selected for DNA binding, which can be tuned by changing environmental factors over physiological ranges including KCl (50-300 mM), MgCl2 (0-10 mM), pH (6.5-8.5) and temperature (23-37 шC). Increasing pH and MgCl2 concentrations switch from Dps-binding to IHF-binding. Stable Dps-DNA and IHF-DNA complexes are insensitive to temperature changes for the range tested. The environment dependent selection between IHF and Dps results in different physical organizations of DNA. Overall, our findings provide important insights into E. coli nucleoid architecture.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175972
ISSN: 2045-2322
DOI: 10.1038/srep18146
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