Please use this identifier to cite or link to this item:
https://doi.org/10.1038/srep18146
DC Field | Value | |
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dc.title | Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF | |
dc.contributor.author | Lee, S.Y | |
dc.contributor.author | Lim, C.J | |
dc.contributor.author | Dr”ge, P | |
dc.contributor.author | Yan, J | |
dc.date.accessioned | 2020-09-14T07:34:29Z | |
dc.date.available | 2020-09-14T07:34:29Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Lee, S.Y, Lim, C.J, Dr”ge, P, Yan, J (2015). Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF. Scientific Reports 5 : 18146. ScholarBank@NUS Repository. https://doi.org/10.1038/srep18146 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/175972 | |
dc.description.abstract | The bacterial nucleoid, a bacterial genome packed by nucleoid binding proteins, forms the physical basis for cellular processes such as gene transcription and DNA replication. Bacteria need to dynamically modulate their nucleoid structures at different growth phases and in response to environmental changes. At the nutrients deficient stationary phase, DNA-binding proteins from starved cells (Dps) and Integration host factors (IHF) are the two most abundant nucleoid associated proteins in E. coli. Yet, it remains unclear how the nucleoid architecture is controlled by the interplay between these two proteins, as well as the nucleoid's response to environmental changes. This question is addressed here using single DNA manipulation approach. Our results reveal that the two proteins are differentially selected for DNA binding, which can be tuned by changing environmental factors over physiological ranges including KCl (50-300 mM), MgCl2 (0-10 mM), pH (6.5-8.5) and temperature (23-37 шC). Increasing pH and MgCl2 concentrations switch from Dps-binding to IHF-binding. Stable Dps-DNA and IHF-DNA complexes are insensitive to temperature changes for the range tested. The environment dependent selection between IHF and Dps results in different physical organizations of DNA. Overall, our findings provide important insights into E. coli nucleoid architecture. | |
dc.source | Unpaywall 20200831 | |
dc.subject | bacterial DNA | |
dc.subject | DNA binding protein | |
dc.subject | Escherichia coli protein | |
dc.subject | integration host factor | |
dc.subject | magnesium chloride | |
dc.subject | potassium chloride | |
dc.subject | protein binding | |
dc.subject | bacterial genome | |
dc.subject | binding competition | |
dc.subject | DNA packaging | |
dc.subject | drug effects | |
dc.subject | Escherichia coli | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | growth, development and aging | |
dc.subject | magnetism | |
dc.subject | metabolism | |
dc.subject | optical tweezers | |
dc.subject | pH | |
dc.subject | Binding, Competitive | |
dc.subject | DNA Packaging | |
dc.subject | DNA, Bacterial | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Escherichia coli | |
dc.subject | Escherichia coli Proteins | |
dc.subject | Gene Expression Regulation, Bacterial | |
dc.subject | Genome, Bacterial | |
dc.subject | Hydrogen-Ion Concentration | |
dc.subject | Integration Host Factors | |
dc.subject | Magnesium Chloride | |
dc.subject | Magnetics | |
dc.subject | Optical Tweezers | |
dc.subject | Potassium Chloride | |
dc.subject | Protein Binding | |
dc.type | Article | |
dc.contributor.department | PHYSICS | |
dc.contributor.department | MECHANOBIOLOGY INSTITUTE | |
dc.description.doi | 10.1038/srep18146 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 5 | |
dc.description.page | 18146 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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10_1038_srep18146.pdf | 3.52 MB | Adobe PDF | OPEN | None | View/Download |
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