Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms10597
Title: NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
Authors: Li, C 
Xue, C
Yang, Q 
Low, B.C 
Liou, Y.-C 
Keywords: cells and cell components
chromosome
protein
vertebrate
chromosome
imaging
kinetochore microtubule
oscillation
velocity
centromere
chromosome
fluorescent antibody technique
HEK293 cell line
HeLa cell line
human
immunoprecipitation
metabolism
metaphase
microtubule
mitosis
movement (physiology)
physiology
spindle apparatus
three dimensional imaging
time lapse imaging
Western blotting
Vertebrata
DNA binding protein
KIF22 protein, human
kinesin
microtubule associated protein
NuSAP protein, human
Blotting, Western
Centromere
Chromosomes
DNA-Binding Proteins
Fluorescent Antibody Technique
HEK293 Cells
HeLa Cells
Humans
Imaging, Three-Dimensional
Immunoprecipitation
Kinesin
Metaphase
Microtubule-Associated Proteins
Microtubules
Mitosis
Movement
Spindle Apparatus
Time-Lapse Imaging
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Li, C, Xue, C, Yang, Q, Low, B.C, Liou, Y.-C (2016). NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nature Communications 7 : 10597. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10597
Abstract: In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation.
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/175432
ISSN: 20411723
DOI: 10.1038/ncomms10597
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