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https://doi.org/10.1038/ncomms10597
| Title: | NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force | Authors: | Li, C Xue, C Yang, Q Low, B.C Liou, Y.-C |
Keywords: | cells and cell components chromosome protein vertebrate chromosome imaging kinetochore microtubule oscillation velocity centromere chromosome fluorescent antibody technique HEK293 cell line HeLa cell line human immunoprecipitation metabolism metaphase microtubule mitosis movement (physiology) physiology spindle apparatus three dimensional imaging time lapse imaging Western blotting Vertebrata DNA binding protein KIF22 protein, human kinesin microtubule associated protein NuSAP protein, human Blotting, Western Centromere Chromosomes DNA-Binding Proteins Fluorescent Antibody Technique HEK293 Cells HeLa Cells Humans Imaging, Three-Dimensional Immunoprecipitation Kinesin Metaphase Microtubule-Associated Proteins Microtubules Mitosis Movement Spindle Apparatus Time-Lapse Imaging |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Li, C, Xue, C, Yang, Q, Low, B.C, Liou, Y.-C (2016). NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nature Communications 7 : 10597. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10597 | Abstract: | In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/175432 | ISSN: | 20411723 | DOI: | 10.1038/ncomms10597 |
| Appears in Collections: | Staff Publications Elements |
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