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Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms10597
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dc.titleNuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
dc.contributor.authorLi, C
dc.contributor.authorXue, C
dc.contributor.authorYang, Q
dc.contributor.authorLow, B.C
dc.contributor.authorLiou, Y.-C
dc.date.accessioned2020-09-10T01:39:23Z
dc.date.available2020-09-10T01:39:23Z
dc.date.issued2016
dc.identifier.citationLi, C, Xue, C, Yang, Q, Low, B.C, Liou, Y.-C (2016). NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nature Communications 7 : 10597. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10597
dc.identifier.issn20411723
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175432
dc.description.abstractIn vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation.
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subjectcells and cell components
dc.subjectchromosome
dc.subjectprotein
dc.subjectvertebrate
dc.subjectchromosome
dc.subjectimaging
dc.subjectkinetochore microtubule
dc.subjectoscillation
dc.subjectvelocity
dc.subjectcentromere
dc.subjectchromosome
dc.subjectfluorescent antibody technique
dc.subjectHEK293 cell line
dc.subjectHeLa cell line
dc.subjecthuman
dc.subjectimmunoprecipitation
dc.subjectmetabolism
dc.subjectmetaphase
dc.subjectmicrotubule
dc.subjectmitosis
dc.subjectmovement (physiology)
dc.subjectphysiology
dc.subjectspindle apparatus
dc.subjectthree dimensional imaging
dc.subjecttime lapse imaging
dc.subjectWestern blotting
dc.subjectVertebrata
dc.subjectDNA binding protein
dc.subjectKIF22 protein, human
dc.subjectkinesin
dc.subjectmicrotubule associated protein
dc.subjectNuSAP protein, human
dc.subjectBlotting, Western
dc.subjectCentromere
dc.subjectChromosomes
dc.subjectDNA-Binding Proteins
dc.subjectFluorescent Antibody Technique
dc.subjectHEK293 Cells
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectImaging, Three-Dimensional
dc.subjectImmunoprecipitation
dc.subjectKinesin
dc.subjectMetaphase
dc.subjectMicrotubule-Associated Proteins
dc.subjectMicrotubules
dc.subjectMitosis
dc.subjectMovement
dc.subjectSpindle Apparatus
dc.subjectTime-Lapse Imaging
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentBIOLOGY (NU)
dc.description.doi10.1038/ncomms10597
dc.description.sourcetitleNature Communications
dc.description.volume7
dc.description.page10597
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