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https://doi.org/10.1128/AAC.00682-13
| Title: | Field-based flow cytometry for ex vivo characterization of plasmodium vivax and P. falciparum antimalarial sensitivity | Authors: | Russell B. Malleret B. Suwanarusk R. Anthony C. Kanlaya S. Lau Y.L. Woodrow C.J. Nosten F. Renia L. |
Keywords: | artesunate chloroquine fluorescent dye hydroethidine antimalarial drug susceptibility article clinical protocol controlled study ex vivo study flow cytometry Giemsa stain human IC 50 major clinical study microscopy parasitemia Plasmodium falciparum Plasmodium vivax priority journal Antimalarials Flow Cytometry Plasmodium falciparum Plasmodium vivax |
Issue Date: | 2013 | Citation: | Russell B., Malleret B., Suwanarusk R., Anthony C., Kanlaya S., Lau Y.L., Woodrow C.J., Nosten F., Renia L. (2013). Field-based flow cytometry for ex vivo characterization of plasmodium vivax and P. falciparum antimalarial sensitivity. Antimicrobial Agents and Chemotherapy 57 (10) : 5170-5174. ScholarBank@NUS Repository. https://doi.org/10.1128/AAC.00682-13 | Abstract: | Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum. Copyright © 2013, American Society for Microbiology. All Rights Reserved. | Source Title: | Antimicrobial Agents and Chemotherapy | URI: | https://scholarbank.nus.edu.sg/handle/10635/175329 | ISSN: | 0066-4804 | DOI: | 10.1128/AAC.00682-13 |
| Appears in Collections: | Staff Publications Elements |
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