Please use this identifier to cite or link to this item: https://doi.org/10.1097/MD.0000000000005263
Title: The transcriptomic G1-G6 signature of hepatocellular carcinoma in an Asian population Association of G3 with microvascular invasion
Authors: Allen, J.C 
Nault, J.-C
Zhu, G
Keat Khor, A.Y 
Liu, J 
Lim, T.K.H 
Zucman-Rossi, J
Chow, P.K.H 
Keywords: alpha fetoprotein
beta catenin
GLUL protein
LGR5 protein
protein
unclassified drug
Wnt protein
adult
Article
blood vessel
clinical feature
comparative study
concentration process
controlled study
disease association
ethnic group
female
gene expression
genetic association
genotype phenotype correlation
hepatitis B
Hepatitis B virus
human
human tissue
liver cell carcinoma
major clinical study
male
population
priority journal
quantitative analysis
reverse transcription polymerase chain reaction
Southeast Asian
transcriptomics
treatment response
aged
Carcinoma, Hepatocellular
classification
ethnology
gene expression profiling
gene expression regulation
genetics
genotype
Liver Neoplasms
middle aged
phenotype
Singapore
tumor gene
tumor invasion
Aged
Carcinoma, Hepatocellular
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes, Neoplasm
Genotype
Humans
Liver Neoplasms
Male
Middle Aged
Neoplasm Invasiveness
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Singapore
Issue Date: 2016
Publisher: Lippincott Williams and Wilkins
Citation: Allen, J.C, Nault, J.-C, Zhu, G, Keat Khor, A.Y, Liu, J, Lim, T.K.H, Zucman-Rossi, J, Chow, P.K.H (2016). The transcriptomic G1-G6 signature of hepatocellular carcinoma in an Asian population Association of G3 with microvascular invasion. Medicine (United States) 95 (47) : e5263. ScholarBank@NUS Repository. https://doi.org/10.1097/MD.0000000000005263
Abstract: In this study, a transcriptomic group classification based on a European population is tested on a Singapore cohort. The results highlight the genotype/phenotype correlation in a Southeast Asian population. The G1-G6 transcriptomic classification derived from hepatocellular carcinoma (HCC) resected from European patients, robustly reflected group-specific clinical/pathological features. We investigated the application of this molecular classification in Southeast Asian HCC patients. Gene expression analysis was carried out on HCC surgically resected in Singapore patients who were grouped into G1-G6 transcriptomic categories according to expression of 16 predictor genes (illustrated in Supplementary Table 1, http://links.lww.com/ MD/B413 and Supplementary Fig. 1, http://links.lww.com/MD/B413) using quantitative reverse transcription polymerase chain reaction (RT-PCR). Univariate and multivariate polytomous logistic regression was used to investigate association between clinical variables and pooled transcriptomic classes G12, G3, and G456. HCC from Singapore (n=82) were distributed (%) into G1 (13.4), G2 (24.4), G3 (15.9), G4 (24.4), G5 (14.6), and G6 (7.3) subgroups. Compared to the European data, the Singapore samples were relatively enriched in G1-G3 versus G4-G6 tumors (53.7% vs 46.3%) reflecting the higher proportion of hepatitis B virus (HBV) patients in Singapore versus Europe samples (43% vs 30%). Pooled classes were defined as G12, G3, and G456. G12 was associated with higher alpha-fetoprotein (AFP) concentrations (OR=1.69, 95% CI: 1.30-2.20; P<0.0001) and G3 with microvascular invasion (OR=4.91, 95% CI: 1.06-24.8; P=0.047). The European and Singapore cohorts were generally similar relative to associations between transcriptomic groups and clinical features. This lends credence to the G1-G6 transcriptomic classifications being applicable regardless of the ethnic origin of HCC patients. The G3 group was associated with microvascular invasion and holds potential for investigation into the underlying mechanisms and selection for therapeutic clinical trials. Copyright © 2016 the Author(s). Published by Wolters Kluwer Health, Inc. All rights reserved.
Source Title: Medicine (United States)
URI: https://scholarbank.nus.edu.sg/handle/10635/175265
ISSN: 0025-7974
DOI: 10.1097/MD.0000000000005263
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