Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.26258
Title: Decoding temporal interpretation of the morphogen bicoid in the early drosophila embryo
Authors: Huang, A 
Amourda, C 
Zhang, S 
Tolwinski, N.S 
Saunders, T.E 
Keywords: morphogen
bicoid protein, Drosophila
homeodomain protein
transactivator protein
animal tissue
Article
blastoderm
cell fate
cell viability
chromatin immunoprecipitation
controlled study
cuticle
Drosophila
embryo
embryo development
female
fertilization
Gap gene
gastrulation
gene
gene expression
gene regulatory network
illumination
immunohistochemistry
nonhuman
optogenetics
real time polymerase chain reaction
transcription regulation
animal
body patterning
Drosophila
embryology
metabolism
survival analysis
time factor
Animals
Body Patterning
Drosophila
Homeodomain Proteins
Optogenetics
Survival Analysis
Time Factors
Trans-Activators
Issue Date: 2017
Citation: Huang, A, Amourda, C, Zhang, S, Tolwinski, N.S, Saunders, T.E (2017). Decoding temporal interpretation of the morphogen bicoid in the early drosophila embryo. eLife 6 : e26258. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.26258
Abstract: Morphogen gradients provide essential spatial information during development. Not only the local concentration but also duration of morphogen exposure is critical for correct cell fate decisions. Yet, how and when cells temporally integrate signals from a morphogen remains unclear. Here, we use optogenetic manipulation to switch off Bicoid-dependent transcription in the early Drosophila embryo with high temporal resolution, allowing time-specific and reversible manipulation of morphogen signalling. We find that Bicoid transcriptional activity is dispensable for embryonic viability in the first hour after fertilization, but persistently required throughout the rest of the blastoderm stage. Short interruptions of Bicoid activity alter the most anterior cell fate decisions, while prolonged inactivation expands patterning defects from anterior to posterior. Such anterior susceptibility correlates with high reliance of anterior gap gene expression on Bicoid. Therefore, cell fates exposed to higher Bicoid concentration require input for longer duration, demonstrating a previously unknown aspect of Bicoid decoding. © Huang et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/175204
ISSN: 2050084X
DOI: 10.7554/eLife.26258
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_7554_eLife_26258.pdf5.78 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

33
checked on Oct 26, 2020

Page view(s)

23
checked on Oct 22, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.