Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.26258
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dc.titleDecoding temporal interpretation of the morphogen bicoid in the early drosophila embryo
dc.contributor.authorHuang, A
dc.contributor.authorAmourda, C
dc.contributor.authorZhang, S
dc.contributor.authorTolwinski, N.S
dc.contributor.authorSaunders, T.E
dc.date.accessioned2020-09-09T05:02:28Z
dc.date.available2020-09-09T05:02:28Z
dc.date.issued2017
dc.identifier.citationHuang, A, Amourda, C, Zhang, S, Tolwinski, N.S, Saunders, T.E (2017). Decoding temporal interpretation of the morphogen bicoid in the early drosophila embryo. eLife 6 : e26258. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.26258
dc.identifier.issn2050084X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175204
dc.description.abstractMorphogen gradients provide essential spatial information during development. Not only the local concentration but also duration of morphogen exposure is critical for correct cell fate decisions. Yet, how and when cells temporally integrate signals from a morphogen remains unclear. Here, we use optogenetic manipulation to switch off Bicoid-dependent transcription in the early Drosophila embryo with high temporal resolution, allowing time-specific and reversible manipulation of morphogen signalling. We find that Bicoid transcriptional activity is dispensable for embryonic viability in the first hour after fertilization, but persistently required throughout the rest of the blastoderm stage. Short interruptions of Bicoid activity alter the most anterior cell fate decisions, while prolonged inactivation expands patterning defects from anterior to posterior. Such anterior susceptibility correlates with high reliance of anterior gap gene expression on Bicoid. Therefore, cell fates exposed to higher Bicoid concentration require input for longer duration, demonstrating a previously unknown aspect of Bicoid decoding. © Huang et al.
dc.sourceUnpaywall 20200831
dc.subjectmorphogen
dc.subjectbicoid protein, Drosophila
dc.subjecthomeodomain protein
dc.subjecttransactivator protein
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectblastoderm
dc.subjectcell fate
dc.subjectcell viability
dc.subjectchromatin immunoprecipitation
dc.subjectcontrolled study
dc.subjectcuticle
dc.subjectDrosophila
dc.subjectembryo
dc.subjectembryo development
dc.subjectfemale
dc.subjectfertilization
dc.subjectGap gene
dc.subjectgastrulation
dc.subjectgene
dc.subjectgene expression
dc.subjectgene regulatory network
dc.subjectillumination
dc.subjectimmunohistochemistry
dc.subjectnonhuman
dc.subjectoptogenetics
dc.subjectreal time polymerase chain reaction
dc.subjecttranscription regulation
dc.subjectanimal
dc.subjectbody patterning
dc.subjectDrosophila
dc.subjectembryology
dc.subjectmetabolism
dc.subjectsurvival analysis
dc.subjecttime factor
dc.subjectAnimals
dc.subjectBody Patterning
dc.subjectDrosophila
dc.subjectHomeodomain Proteins
dc.subjectOptogenetics
dc.subjectSurvival Analysis
dc.subjectTime Factors
dc.subjectTrans-Activators
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentYALE-NUS COLLEGE
dc.contributor.departmentBIOLOGY (NU)
dc.description.doi10.7554/eLife.26258
dc.description.sourcetitleeLife
dc.description.volume6
dc.description.pagee26258
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