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https://doi.org/10.1038/srep28876
Title: | A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema | Authors: | Victorio, C.B.L Xu, Y Ng, Q Chua, B.H Alonso, S Chow, V.T.K Chua, K.B |
Keywords: | catecholamine neutralizing antibody animal Bagg albino mouse blood brain disease model Enterovirus A Enterovirus infection human immunology Kaplan Meier method lung lung edema metabolism mortality mouse pathology physiology severity of illness index virology Animals Antibodies, Neutralizing Brain Catecholamines Disease Models, Animal Enterovirus A, Human Enterovirus Infections Humans Kaplan-Meier Estimate Lung Mice Mice, Inbred BALB C Pulmonary Edema Severity of Illness Index |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Victorio, C.B.L, Xu, Y, Ng, Q, Chua, B.H, Alonso, S, Chow, V.T.K, Chua, K.B (2016). A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema. Scientific Reports 6 : 28876. ScholarBank@NUS Repository. https://doi.org/10.1038/srep28876 | Abstract: | Enterovirus 71 (EV-A71) is a neurotropic virus that sporadically causes fatal neurologic illness among infected children. Animal models of EV-A71 infection exist, but they do not recapitulate in animals the spectrum of disease and pathology observed in fatal human cases. Specifically, neurogenic pulmonary oedema (NPE) - the main cause of EV-A71 infection-related mortality - is not observed in any of these models. This limits their utility in understanding viral pathogenesis of neurologic infections. We report the development of a mouse model of EV-A71 infection displaying NPE in severely affected animals. We inoculated one-week-old BALB/c mice with an adapted EV-A71 strain and identified clinical signs consistent with observations in human cases and other animal models. We also observed respiratory distress in some mice. At necropsy, we found their lungs to be heavier and incompletely collapsed compared to other mice. Serum levels of catecholamines and histopathology of lung and brain tissues of these mice strongly indicated onset of NPE. The localization of virally-induced brain lesions also suggested a potential pathogenic mechanism for EV-A71-induced NPE. This novel mouse model of virally-induced NPE represents a valuable resource for studying viral mechanisms of neuro-pathogenesis and pre-clinical testing of potential therapeutics and prophylactics against EV-A71-related neurologic complications. | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/174951 | ISSN: | 20452322 | DOI: | 10.1038/srep28876 |
Appears in Collections: | Elements Staff Publications |
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