Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-017-00172-9
Title: An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins
Authors: Li S. 
Koe C.T. 
Tay S.T. 
Tan A.L.K. 
Zhang S. 
Zhang Y. 
Tan P. 
Sung W.-K. 
Wang H. 
Keywords: insulin
matrix protein
phosphatidylinositol 3 kinase
protein Chromator
somatomedin
transcription factor
transcription factor Grainy head
transcription factor Prospero
unclassified drug
chromator protein, Drosophila
DNA binding protein
Drosophila protein
EAST protein, Drosophila
grh protein, Drosophila
megator protein, Drosophila
nerve protein
nuclear matrix protein
nuclear protein
phosphoprotein
pros protein, Drosophila
transcription factor
biochemistry
biological development
cells and cell components
gene expression
growth
hormone
nervous system disorder
physiology
protein
reactivation
animal cell
animal tissue
Article
cell proliferation
controlled study
Drosophila
neural stem cell
nonhuman
protein function
signal transduction
animal
confocal microscopy
cytology
Drosophila melanogaster
gene expression profiling
genetics
larva
metabolism
neural stem cell
procedures
RNA interference
transgenic animal
Western blotting
Prospero
Animals
Animals, Genetically Modified
Blotting, Western
DNA-Binding Proteins
Drosophila melanogaster
Drosophila Proteins
Gene Expression Profiling
Larva
Microscopy, Confocal
Nerve Tissue Proteins
Neural Stem Cells
Nuclear Matrix-Associated Proteins
Nuclear Proteins
Phosphoproteins
RNA Interference
Transcription Factors
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Li S., Koe C.T., Tay S.T., Tan A.L.K., Zhang S., Zhang Y., Tan P., Sung W.-K., Wang H. (2017). An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins. Nature Communications 8 (1) : 122. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00172-9
Abstract: The switch between quiescence and proliferation is central for neurogenesis and its alteration is linked to neurodevelopmental disorders such as microcephaly. However, intrinsic mechanisms that reactivate Drosophila larval neural stem cells (NSCs) to exit from quiescence are not well established. Here we show that the spindle matrix complex containing Chromator (Chro) functions as a key intrinsic regulator of NSC reactivation downstream of extrinsic insulin/insulin-like growth factor signalling. Chro also prevents NSCs from ire-entering quiescence at later stages. NSC-specific in vivo profiling has dentified many downstream targets of Chro, including a temporal transcription factor Grainy head (Grh) and a neural stem cell quiescence-inducing factor Prospero (Pros). We show that spindle matrix proteins promote the expression of Grh and repress that of Pros in NSCs to govern their reactivation. Our data demonstrate that nuclear Chro critically regulates gene expression in NSCs at the transition from quiescence to proliferation. © 2017 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174488
ISSN: 2041-1723
DOI: 10.1038/s41467-017-00172-9
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