Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-017-00172-9
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dc.titleAn intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins
dc.contributor.authorLi S.
dc.contributor.authorKoe C.T.
dc.contributor.authorTay S.T.
dc.contributor.authorTan A.L.K.
dc.contributor.authorZhang S.
dc.contributor.authorZhang Y.
dc.contributor.authorTan P.
dc.contributor.authorSung W.-K.
dc.contributor.authorWang H.
dc.date.accessioned2020-09-06T16:04:25Z
dc.date.available2020-09-06T16:04:25Z
dc.date.issued2017
dc.identifier.citationLi S., Koe C.T., Tay S.T., Tan A.L.K., Zhang S., Zhang Y., Tan P., Sung W.-K., Wang H. (2017). An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins. Nature Communications 8 (1) : 122. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00172-9
dc.identifier.issn2041-1723
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174488
dc.description.abstractThe switch between quiescence and proliferation is central for neurogenesis and its alteration is linked to neurodevelopmental disorders such as microcephaly. However, intrinsic mechanisms that reactivate Drosophila larval neural stem cells (NSCs) to exit from quiescence are not well established. Here we show that the spindle matrix complex containing Chromator (Chro) functions as a key intrinsic regulator of NSC reactivation downstream of extrinsic insulin/insulin-like growth factor signalling. Chro also prevents NSCs from ire-entering quiescence at later stages. NSC-specific in vivo profiling has dentified many downstream targets of Chro, including a temporal transcription factor Grainy head (Grh) and a neural stem cell quiescence-inducing factor Prospero (Pros). We show that spindle matrix proteins promote the expression of Grh and repress that of Pros in NSCs to govern their reactivation. Our data demonstrate that nuclear Chro critically regulates gene expression in NSCs at the transition from quiescence to proliferation. © 2017 The Author(s).
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subjectinsulin
dc.subjectmatrix protein
dc.subjectphosphatidylinositol 3 kinase
dc.subjectprotein Chromator
dc.subjectsomatomedin
dc.subjecttranscription factor
dc.subjecttranscription factor Grainy head
dc.subjecttranscription factor Prospero
dc.subjectunclassified drug
dc.subjectchromator protein, Drosophila
dc.subjectDNA binding protein
dc.subjectDrosophila protein
dc.subjectEAST protein, Drosophila
dc.subjectgrh protein, Drosophila
dc.subjectmegator protein, Drosophila
dc.subjectnerve protein
dc.subjectnuclear matrix protein
dc.subjectnuclear protein
dc.subjectphosphoprotein
dc.subjectpros protein, Drosophila
dc.subjecttranscription factor
dc.subjectbiochemistry
dc.subjectbiological development
dc.subjectcells and cell components
dc.subjectgene expression
dc.subjectgrowth
dc.subjecthormone
dc.subjectnervous system disorder
dc.subjectphysiology
dc.subjectprotein
dc.subjectreactivation
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectDrosophila
dc.subjectneural stem cell
dc.subjectnonhuman
dc.subjectprotein function
dc.subjectsignal transduction
dc.subjectanimal
dc.subjectconfocal microscopy
dc.subjectcytology
dc.subjectDrosophila melanogaster
dc.subjectgene expression profiling
dc.subjectgenetics
dc.subjectlarva
dc.subjectmetabolism
dc.subjectneural stem cell
dc.subjectprocedures
dc.subjectRNA interference
dc.subjecttransgenic animal
dc.subjectWestern blotting
dc.subjectProspero
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectBlotting, Western
dc.subjectDNA-Binding Proteins
dc.subjectDrosophila melanogaster
dc.subjectDrosophila Proteins
dc.subjectGene Expression Profiling
dc.subjectLarva
dc.subjectMicroscopy, Confocal
dc.subjectNerve Tissue Proteins
dc.subjectNeural Stem Cells
dc.subjectNuclear Matrix-Associated Proteins
dc.subjectNuclear Proteins
dc.subjectPhosphoproteins
dc.subjectRNA Interference
dc.subjectTranscription Factors
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentDEPARTMENT OF COMPUTER SCIENCE
dc.description.doi10.1038/s41467-017-00172-9
dc.description.sourcetitleNature Communications
dc.description.volume8
dc.description.issue1
dc.description.page122
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