Please use this identifier to cite or link to this item:
https://doi.org/10.18632/oncotarget.5636
Title: | TIP60-miR-22 axis as a prognostic marker of breast cancer progression | Authors: | Pandey, A.K Zhang, Y Zhang, S Li, Y Tucker-Kellogg, G Yang, H Jha, S |
Keywords: | acyltransferase messenger RNA microRNA 22 protein TIP60 unclassified drug 3' untranslated region histone acetyltransferase KAT5 protein, human microRNA MIRN22 microRNA, human tumor marker 3' untranslated region Article breast cancer breast cancer cell line cancer growth cancer patient cancer prognosis cell invasion controlled study disease association epithelial mesenchymal transition gene expression genetic transfection human human cell migration inhibition overall survival protein analysis protein expression protein targeting breast tumor cell line cell motion confocal microscopy disease course female gene expression regulation genetics HEK293 cell line Kaplan Meier method MCF-7 cell line metabolism pathology prognosis reverse transcription polymerase chain reaction tumor cell line 3' Untranslated Regions Biomarkers, Tumor Breast Neoplasms Cell Line Cell Line, Tumor Cell Movement Disease Progression Epithelial-Mesenchymal Transition Female Gene Expression Regulation, Neoplastic HEK293 Cells Histone Acetyltransferases Humans Kaplan-Meier Estimate MCF-7 Cells MicroRNAs Microscopy, Confocal Prognosis Reverse Transcriptase Polymerase Chain Reaction |
Issue Date: | 2015 | Citation: | Pandey, A.K, Zhang, Y, Zhang, S, Li, Y, Tucker-Kellogg, G, Yang, H, Jha, S (2015). TIP60-miR-22 axis as a prognostic marker of breast cancer progression. Oncotarget 6 (38) : 41290-41306. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.5636 | Abstract: | MicroRNAs (miRNAs) are 22- to 24-nucleotide, small, non-coding RNAs that bind to the 3'UTR of target genes to control gene expression. Consequently, their dysregulation contributes to many diseases, including diabetes and cancer. miR-22 is up-regulated in numerous metastatic cancers and recent studies have suggested a role for miR-22 in promoting stemness and metastasis. TIP60 is a lysine acetyltransferase reported to be down-regulated in cancer but the molecular mechanism of this reduction is still unclear. In this study, we identify TIP60 as a target of miR-22. We show a negative correlation in the expression of TIP60 and miR-22 in breast cancer patients, and show that low levels of TIP60 and high levels of miR-22 are associated with poor overall survival. Furthermore, pathway analysis using high miR-22/low TIP60 and low miR-22/high TIP60 breast cancer patient datasets suggests association of TIP60/miR-22 with epithelial-mesenchymal transition (EMT), a key alteration in progression of cancer cells. We show that blocking endogenous miR-22 can restore TIP60 levels, which in turn decreases the migration and invasion capacity of metastatic breast cancer cell line. These results provide mechanistic insight into TIP60 regulation and evidence for the utility of the combination of TIP60 and miR-22 as prognostic indicator of breast cancer progression. | Source Title: | Oncotarget | URI: | https://scholarbank.nus.edu.sg/handle/10635/174130 | ISSN: | 19492553 | DOI: | 10.18632/oncotarget.5636 |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_18632_oncotarget_5636.pdf | 5.75 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.