Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep20632
Title: Silver nanoparticles disrupt germline stem cell maintenance in the Drosophila testis
Authors: Ong, C
Lee, Q.Y
Cai, Y 
Liu, X 
Ding, J 
Yung, L.-Y.L 
Bay, B.-H 
Baeg, G.-H 
Keywords: metal nanoparticle
reactive oxygen metabolite
silver
animal
cell differentiation
cell survival
chemistry
cytology
dose response
Drosophila
drug effects
fertility
germ cell
growth, development and aging
male
metabolism
testis
Animals
Cell Differentiation
Cell Survival
Dose-Response Relationship, Drug
Drosophila
Fertility
Germ Cells
Male
Metal Nanoparticles
Reactive Oxygen Species
Silver
Testis
Issue Date: 2016
Citation: Ong, C, Lee, Q.Y, Cai, Y, Liu, X, Ding, J, Yung, L.-Y.L, Bay, B.-H, Baeg, G.-H (2016). Silver nanoparticles disrupt germline stem cell maintenance in the Drosophila testis. Scientific Reports 6 : 20632. ScholarBank@NUS Repository. https://doi.org/10.1038/srep20632
Abstract: Silver nanoparticles (AgNPs), one of the most popular nanomaterials, are commonly used in consumer products and biomedical devices, despite their potential toxicity. Recently, AgNP exposure was reported to be associated with male reproductive toxicity in mammalian models. However, there is still a limited understanding of the effects of AgNPs on spermatogenesis. The fruit fly Drosophila testis is an excellent in vivo model to elucidate the mechanisms underlying AgNP-induced defects in spermatogenesis, as germ lineages can be easily identified and imaged. In this study, we evaluated AgNP-mediated toxicity on spermatogenesis by feeding Drosophila with AgNPs at various concentrations. We first observed a dose-dependent uptake of AgNPs in vivo. Concomitantly, AgNP exposure caused a significant decrease in the viability and delay in the development of Drosophila in a dose-dependent manner. Furthermore, AgNP-treated male flies showed a reduction in fecundity, and the resulting testes contained a decreased number of germline stem cells (GSCs) compared to controls. Interestingly, testes exposed to AgNPs exhibited a dramatic increase in reactive oxygen species levels and showed precocious GSC differentiation. Taken together, our study suggests that AgNP exposure may increase ROS levels in the Drosophila testis, leading to a reduction of GSC number by promoting premature GSC differentiation.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174027
ISSN: 20452322
DOI: 10.1038/srep20632
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