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|Title:||A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection||Authors:||Dung T.T.N.
Hong Chau T.T.
nucleic acid amplification
randomized controlled trial (topic)
Sequence Analysis, RNA
|Issue Date:||2017||Publisher:||BioMed Central Ltd.||Citation:||Dung T.T.N., Duy P.T., Sessions O.M., Sangumathi U.K., Phat V.V., Tam P.T.T., To N.T.N., Phuc T.M., Hong Chau T.T., Chau N.N.M., Minh N.N., Thwaites G.E., Rabaa M.A., Baker S. (2017). A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection. BMC Genomics 18 (1) : 324. ScholarBank@NUS Repository. https://doi.org/10.1186/s12864-017-3714-6||Abstract:||Background: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. Methods: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents. Results: Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30). Conclusions: This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems. Trial registration (prospectively registered): Current Controlled Trials ISRCTN88101063. Date of registration: 14/06/2012 © 2017 The Author(s).||Source Title:||BMC Genomics||URI:||https://scholarbank.nus.edu.sg/handle/10635/173848||ISSN:||14712164||DOI:||10.1186/s12864-017-3714-6|
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