Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12864-017-3714-6
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dc.titleA universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection
dc.contributor.authorDung T.T.N.
dc.contributor.authorDuy P.T.
dc.contributor.authorSessions O.M.
dc.contributor.authorSangumathi U.K.
dc.contributor.authorPhat V.V.
dc.contributor.authorTam P.T.T.
dc.contributor.authorTo N.T.N.
dc.contributor.authorPhuc T.M.
dc.contributor.authorHong Chau T.T.
dc.contributor.authorChau N.N.M.
dc.contributor.authorMinh N.N.
dc.contributor.authorThwaites G.E.
dc.contributor.authorRabaa M.A.
dc.contributor.authorBaker S.
dc.date.accessioned2020-09-01T07:55:34Z
dc.date.available2020-09-01T07:55:34Z
dc.date.issued2017
dc.identifier.citationDung T.T.N., Duy P.T., Sessions O.M., Sangumathi U.K., Phat V.V., Tam P.T.T., To N.T.N., Phuc T.M., Hong Chau T.T., Chau N.N.M., Minh N.N., Thwaites G.E., Rabaa M.A., Baker S. (2017). A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection. BMC Genomics 18 (1) : 324. ScholarBank@NUS Repository. https://doi.org/10.1186/s12864-017-3714-6
dc.identifier.issn14712164
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173848
dc.description.abstractBackground: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. Methods: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents. Results: Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30). Conclusions: This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems. Trial registration (prospectively registered): Current Controlled Trials ISRCTN88101063. Date of registration: 14/06/2012 © 2017 The Author(s).
dc.publisherBioMed Central Ltd.
dc.sourceUnpaywall 20200831
dc.subjectArticle
dc.subjectchild
dc.subjectenzyme immunoassay
dc.subjectfeces analysis
dc.subjectgene amplification
dc.subjectgenetic reassortment
dc.subjecthuman
dc.subjectmixed infection
dc.subjectnonhuman
dc.subjectnucleic acid amplification
dc.subjectnucleotide sequence
dc.subjectphylogeny
dc.subjectrandomized controlled trial (topic)
dc.subjectRNA extraction
dc.subjectRotavirus A
dc.subjectRotavirus infection
dc.subjectsequence analysis
dc.subjectunindexed sequence
dc.subjectvirus genome
dc.subjectvirus load
dc.subjectvirus purification
dc.subjectfeces
dc.subjectgenetic reassortment
dc.subjectgenetics
dc.subjectgenomics
dc.subjectgenotype
dc.subjectphysiology
dc.subjectprocedures
dc.subjectRotavirus
dc.subjectvirology
dc.subjectvirus genome
dc.subjectcomplementary DNA
dc.subjectDNA, Complementary
dc.subjectFeces
dc.subjectGenome, Viral
dc.subjectGenomics
dc.subjectGenotype
dc.subjectHumans
dc.subjectPhylogeny
dc.subjectReassortant Viruses
dc.subjectRotavirus
dc.subjectSequence Analysis, RNA
dc.subjectViral Load
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1186/s12864-017-3714-6
dc.description.sourcetitleBMC Genomics
dc.description.volume18
dc.description.issue1
dc.description.page324
dc.published.statePublished
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