Please use this identifier to cite or link to this item: https://doi.org/10.1186/s40793-016-0175-5
Title: Insights from the draft genome of the subsection V (Stigonematales) cyanobacterium Hapalosiphon sp. Strain MRB220 associated with 2-MIB production
Authors: Tan, B.F
Te, S.H 
Boo, C.Y 
Gin, K.Y.-H 
Thompson, J.R
Keywords: 2 methylisoborneol
allophycocyanin
chlorophyll a
geosmin
nitrogen
phycocyanin
phycoerythrin
RNA 16S
Article
bacterial genome
bioinformatics
biomass production
controlled study
cyanobacterium
Fischerella
genetic association
genome analysis
Hapalosiphon
mass fragmentography
metabolomics
nitrogen fixation
nonhuman
nucleotide sequence
phylogeny
sequence alignment
sequence analysis
sequence homology
solid phase microextraction
Stigonematales
Synechocystis
Issue Date: 2016
Citation: Tan, B.F, Te, S.H, Boo, C.Y, Gin, K.Y.-H, Thompson, J.R (2016). Insights from the draft genome of the subsection V (Stigonematales) cyanobacterium Hapalosiphon sp. Strain MRB220 associated with 2-MIB production. Standards in Genomic Sciences 11 (1) : 58. ScholarBank@NUS Repository. https://doi.org/10.1186/s40793-016-0175-5
Abstract: A non-axenic unialgal culture containing a Subsection V (Stigonematales) cyanobacterium, Hapalosiphon strain MRB 220, was obtained from a benthic freshwater algal mat through multiple transfers following growth in sterile media. Physiological characterization demonstrated the culture was capable of nitrogen-fixation and production of the off flavor compound 2-methylisoborneol (2-MIB). Total DNA isolated from this culture was sequenced using Illumina HiSeq and de novo assembled into contigs. The genome of MRB 220 was separated from co-occurring heterotrophic bacteria using sequence homology and compositional approaches, and its purity was confirmed based on best BLAST hit classification and principle component analysis of the tetranucleotide frequencies of fragmented contigs. The genome of ~7.4 Mbp contains 6,345 protein coding genes with 4,320 of these having functional prediction including predicted pathways for biosynthesis of the secondary metabolite welwitindolinone. Analyses of 16S rRNA gene and whole genome sequence average nucleotide identity indicated close relatedness of MRB 220 to the genera Hapalosiphon and Fischerella within the order Stigonematales. Microscopic examination showed that MRB 220 formed heterocystous branched filaments, thereby supporting identification of strain MRB 220 as a morphospecies of Hapalosiphon. Availability of the draft genome of Hapalosiphon strain MRB 220 enables future work to elucidate the pathway and dynamics for biosynthesis of 2-MIB and other secondary metabolites and understand the ecology and physiology of Stigonematales cyanobacteria in tropical freshwaters. © 2016 The Author(s).
Source Title: Standards in Genomic Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/173821
ISSN: 19443277
DOI: 10.1186/s40793-016-0175-5
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s40793-016-0175-5.pdf1.98 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

4
checked on Sep 29, 2022

Page view(s)

176
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.