Please use this identifier to cite or link to this item: https://doi.org/10.1186/s40793-016-0175-5
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dc.titleInsights from the draft genome of the subsection V (Stigonematales) cyanobacterium Hapalosiphon sp. Strain MRB220 associated with 2-MIB production
dc.contributor.authorTan, B.F
dc.contributor.authorTe, S.H
dc.contributor.authorBoo, C.Y
dc.contributor.authorGin, K.Y.-H
dc.contributor.authorThompson, J.R
dc.date.accessioned2020-09-01T01:02:36Z
dc.date.available2020-09-01T01:02:36Z
dc.date.issued2016
dc.identifier.citationTan, B.F, Te, S.H, Boo, C.Y, Gin, K.Y.-H, Thompson, J.R (2016). Insights from the draft genome of the subsection V (Stigonematales) cyanobacterium Hapalosiphon sp. Strain MRB220 associated with 2-MIB production. Standards in Genomic Sciences 11 (1) : 58. ScholarBank@NUS Repository. https://doi.org/10.1186/s40793-016-0175-5
dc.identifier.issn19443277
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173821
dc.description.abstractA non-axenic unialgal culture containing a Subsection V (Stigonematales) cyanobacterium, Hapalosiphon strain MRB 220, was obtained from a benthic freshwater algal mat through multiple transfers following growth in sterile media. Physiological characterization demonstrated the culture was capable of nitrogen-fixation and production of the off flavor compound 2-methylisoborneol (2-MIB). Total DNA isolated from this culture was sequenced using Illumina HiSeq and de novo assembled into contigs. The genome of MRB 220 was separated from co-occurring heterotrophic bacteria using sequence homology and compositional approaches, and its purity was confirmed based on best BLAST hit classification and principle component analysis of the tetranucleotide frequencies of fragmented contigs. The genome of ~7.4 Mbp contains 6,345 protein coding genes with 4,320 of these having functional prediction including predicted pathways for biosynthesis of the secondary metabolite welwitindolinone. Analyses of 16S rRNA gene and whole genome sequence average nucleotide identity indicated close relatedness of MRB 220 to the genera Hapalosiphon and Fischerella within the order Stigonematales. Microscopic examination showed that MRB 220 formed heterocystous branched filaments, thereby supporting identification of strain MRB 220 as a morphospecies of Hapalosiphon. Availability of the draft genome of Hapalosiphon strain MRB 220 enables future work to elucidate the pathway and dynamics for biosynthesis of 2-MIB and other secondary metabolites and understand the ecology and physiology of Stigonematales cyanobacteria in tropical freshwaters. © 2016 The Author(s).
dc.sourceUnpaywall 20200831
dc.subject2 methylisoborneol
dc.subjectallophycocyanin
dc.subjectchlorophyll a
dc.subjectgeosmin
dc.subjectnitrogen
dc.subjectphycocyanin
dc.subjectphycoerythrin
dc.subjectRNA 16S
dc.subjectArticle
dc.subjectbacterial genome
dc.subjectbioinformatics
dc.subjectbiomass production
dc.subjectcontrolled study
dc.subjectcyanobacterium
dc.subjectFischerella
dc.subjectgenetic association
dc.subjectgenome analysis
dc.subjectHapalosiphon
dc.subjectmass fragmentography
dc.subjectmetabolomics
dc.subjectnitrogen fixation
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectphylogeny
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectsequence homology
dc.subjectsolid phase microextraction
dc.subjectStigonematales
dc.subjectSynechocystis
dc.typeArticle
dc.contributor.departmentNUS ENVIRONMENTAL RESEARCH INSTITUTE
dc.contributor.departmentDEPT OF CIVIL & ENVIRONMENTAL ENGG
dc.description.doi10.1186/s40793-016-0175-5
dc.description.sourcetitleStandards in Genomic Sciences
dc.description.volume11
dc.description.issue1
dc.description.page58
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