Please use this identifier to cite or link to this item: https://doi.org/10.3389/fimmu.2018.02514
Title: A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium
Authors: Luukkainen A. 
Puan K.J.
Yusof N.
Lee B.
Tan K.S.
Liu J. 
Yan Y. 
Toppila-Salmi S.
Renkonen R.
Chow V.T. 
Rotzschke O.
Wang Y. 
Keywords: chemokine
cytokine
interferon
cell culture
coculture
human
immunology
influenza
Influenza A virus (H3N2)
innate immunity
male
middle aged
monocyte
mononuclear cell
natural killer cell
nose mucosa
procedures
stem cell
T lymphocyte
virology
Cells, Cultured
Chemokines
Coculture Techniques
Cytokines
Humans
Immunity, Innate
Influenza A Virus, H3N2 Subtype
Influenza, Human
Interferons
Killer Cells, Natural
Leukocytes, Mononuclear
Male
Middle Aged
Monocytes
Nasal Mucosa
Stem Cells
T-Lymphocytes
Issue Date: 2018
Citation: Luukkainen A., Puan K.J., Yusof N., Lee B., Tan K.S., Liu J., Yan Y., Toppila-Salmi S., Renkonen R., Chow V.T., Rotzschke O., Wang Y. (2018). A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium. Frontiers in immunology 9 : 2514. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2018.02514
Abstract: Background: We established an in vitro co-culture model involving H3N2-infection of human nasal epithelium with peripheral blood mononuclear cells (PBMC) to investigate their cross-talk during early H3N2 infection. Methods: Nasal epithelium was differentiated from human nasal epithelial stem/progenitor cells and cultured wtih fresh human PBMC. PBMC and supernatants were harvested after 24 and 48 h of co-culture with H3N2-infected nasal epithelium. We used flow cytometry and Luminex to characterize PBMC subpopulations, their activation and secretion of cytokine and chemokines. Results: H3N2 infection of the nasal epithelium associated with significant increase in interferons (IFN-?, IFN-?, IL-29), pro-inflammatory cytokines (TNF-?, BDNF, IL-3) and viral-associated chemokines (IP-10, MCP-3, I-TAC, MIG), detectable already after 24 h. This translates into rapid activation of monocytes, NK-cells and innate T-cells (MAIT and ?? T cells), evident with CD38+ and/or CD69+ upregulation. Conclusions: This system may contribute to in vitro mechanistic immunological studies bridging systemic models and possibly enable the development of targeted immunomodulatory therapies.
Source Title: Frontiers in immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/173747
ISSN: 16643224
DOI: 10.3389/fimmu.2018.02514
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