Please use this identifier to cite or link to this item: https://doi.org/10.3389/fimmu.2018.02514
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dc.titleA Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium
dc.contributor.authorLuukkainen A.
dc.contributor.authorPuan K.J.
dc.contributor.authorYusof N.
dc.contributor.authorLee B.
dc.contributor.authorTan K.S.
dc.contributor.authorLiu J.
dc.contributor.authorYan Y.
dc.contributor.authorToppila-Salmi S.
dc.contributor.authorRenkonen R.
dc.contributor.authorChow V.T.
dc.contributor.authorRotzschke O.
dc.contributor.authorWang Y.
dc.date.accessioned2020-09-01T00:46:38Z
dc.date.available2020-09-01T00:46:38Z
dc.date.issued2018
dc.identifier.citationLuukkainen A., Puan K.J., Yusof N., Lee B., Tan K.S., Liu J., Yan Y., Toppila-Salmi S., Renkonen R., Chow V.T., Rotzschke O., Wang Y. (2018). A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium. Frontiers in immunology 9 : 2514. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2018.02514
dc.identifier.issn16643224
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173747
dc.description.abstractBackground: We established an in vitro co-culture model involving H3N2-infection of human nasal epithelium with peripheral blood mononuclear cells (PBMC) to investigate their cross-talk during early H3N2 infection. Methods: Nasal epithelium was differentiated from human nasal epithelial stem/progenitor cells and cultured wtih fresh human PBMC. PBMC and supernatants were harvested after 24 and 48 h of co-culture with H3N2-infected nasal epithelium. We used flow cytometry and Luminex to characterize PBMC subpopulations, their activation and secretion of cytokine and chemokines. Results: H3N2 infection of the nasal epithelium associated with significant increase in interferons (IFN-?, IFN-?, IL-29), pro-inflammatory cytokines (TNF-?, BDNF, IL-3) and viral-associated chemokines (IP-10, MCP-3, I-TAC, MIG), detectable already after 24 h. This translates into rapid activation of monocytes, NK-cells and innate T-cells (MAIT and ?? T cells), evident with CD38+ and/or CD69+ upregulation. Conclusions: This system may contribute to in vitro mechanistic immunological studies bridging systemic models and possibly enable the development of targeted immunomodulatory therapies.
dc.sourceUnpaywall 20200831
dc.subjectchemokine
dc.subjectcytokine
dc.subjectinterferon
dc.subjectcell culture
dc.subjectcoculture
dc.subjecthuman
dc.subjectimmunology
dc.subjectinfluenza
dc.subjectInfluenza A virus (H3N2)
dc.subjectinnate immunity
dc.subjectmale
dc.subjectmiddle aged
dc.subjectmonocyte
dc.subjectmononuclear cell
dc.subjectnatural killer cell
dc.subjectnose mucosa
dc.subjectprocedures
dc.subjectstem cell
dc.subjectT lymphocyte
dc.subjectvirology
dc.subjectCells, Cultured
dc.subjectChemokines
dc.subjectCoculture Techniques
dc.subjectCytokines
dc.subjectHumans
dc.subjectImmunity, Innate
dc.subjectInfluenza A Virus, H3N2 Subtype
dc.subjectInfluenza, Human
dc.subjectInterferons
dc.subjectKiller Cells, Natural
dc.subjectLeukocytes, Mononuclear
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMonocytes
dc.subjectNasal Mucosa
dc.subjectStem Cells
dc.subjectT-Lymphocytes
dc.typeArticle
dc.contributor.departmentDEPT OF OTOLARYNGOLOGY
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.description.doi10.3389/fimmu.2018.02514
dc.description.sourcetitleFrontiers in immunology
dc.description.volume9
dc.description.page2514
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